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  • A3813 - Anti-Human Kappa Light Chains (Bound and Free)−Alkaline Phosphatase antibody produced in goat

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A3813 Sigma

Anti-Human Kappa Light Chains (Bound and Free)−Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous glycerol solution

Properties

Related Categories Alkaline Phosphatase, Alphabetical Index, Antibodies, HU-HZ, Human Kappa Secondary Antibodies and Conjugates,
species reactivity   human
application(s)   direct ELISA: 1:30,000
  dot blot: 1:30,000
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous glycerol solution
shipped in   wet ice
storage temp.   2-8°C
Gene Information   human ... IGK@(50802)
biological source   goat
conjugate   alkaline phosphatase conjugate

Description

Immunogen

Purified Bence Jones κ light chain

General description

Human κ light chains are antibody fragments that regulate immunological responses in cells. Increased expression of κ light chains has been linked to chronic lymphocytic leukemia . The variable fragment of the κ light chain gene has also been associated with vaccine-induced antibody response to the polysaccharide coat in Haemophilus influenzae type b . Anti-Human κ Light Chains (Bound and Free-Alkaline Phosphatase antibody is specific for human κ light chain when tested against human IgA, IgG, IgM, IgGλ, IgAλ, IgMλ, Bence Jones κ and λ myeloma proteins.

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide.

Application

Anti-Human κ Light Chains (Bound and Free)-Alkaline Phosphatase antibody is suitable for use in ELISA. The product can also be used for dot blot (1:30,000) and immunohistochemistry (1:50 using formalin-fixed, paraffin-embedded sections).

Mouse-Human chimeric IgG′s specific for Pseudomonas aeruginosa serogroup 06 lipopolysaccharide were identified by ELISA in transfected CHO cell supernatants using Alkaline phosphatase conjugated goat anti-Human Kappa Light chains.

Price and Availability


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