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A3860 Sigma

Anti-APBB1 (401-415) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-Amyloid β (A4) precursor protein-binding, family B, member 1 (Fe65), Anti-FE65, Anti-RIR

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Properties

Related Categories AO-AQ, Alphabetical Index, Antibodies, Primary Antibodies
species reactivity   human
application(s)   western blot: 1:500-1:2,000
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt ~77 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... APBB1(322)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 401-415 of human APBB1

General description

APBB1 or FE65 is an adaptor protein that binds to the beta-amyloid precursor protein (APP) and Tip60. Studies have reported that APBB1 may play a role in nuclear signaling by forming APP-APBB1-Tip60 complexes. Rabbit anti-APBB1 (401-415) antibody binds to human APBB1.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Rabbit anti-APBB1 was used to detect amyloid expression in mouse nasal tissue by western blot and ICC of frozen tissue. The antibody was used at a dilution of 1:1000. APBB1 was found expressed in GnRh1 neurons.

Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about the Sigma Life Science and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

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