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A3861 Sigma

Anti-Palladin (C-terminal) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-PALLD

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Actin-associated Proteins/Myosin, Antibodies to Cell and Organelle Proteins,
species reactivity   human
application(s)   immunoprecipitation: 10-20 μL using HeLa cell lysates
  indirect immunofluorescence: 1:250-1:500 using human HeLa cells
  western blot: 1:250-1:500 using whole extracts of human HeLa cells
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt ~140 kDa (another band at 90 kDa)
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... PALLD(23022)
mouse ... Palld(72333)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 1053-1066 of human palladin, conjugated to KLH. The corresponding sequence is identical in rat and differs by 1 amino acid in mouse.

General description

Palladin is a microfilament-associated phosphoprotein that is known to colocalize with alpha-actinin at cell-cell junctions, stress fibres, embryonic Z-lines, and focal adhesions. Palladin abnormalities have been linked to familial pancreatic cancer. Rabbit anti-Palladin (C-terminal) antibody binds to human palladin. Detection of the palladin band by immunoblotting is specifically inhibited by the immunizing peptide. A nonspecific band of approx. 55kDa may be detected in some cell extract preparations.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Rabbit anti-Palladin (C-terminal) antibody can be used for immunoprecipitation (10-20μL), indirect immunofluorescence (1:250-1:500) and western blot (1:250 -1:500) using HeLa cells.

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WGK Germany  1
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