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  • A3963 - Monoclonal Anti-Maltose Binding Protein−Alkaline Phosphatase antibody produced in mouse

A3963 Sigma

Monoclonal Anti-Maltose Binding Protein−Alkaline Phosphatase antibody produced in mouse

clone MBP-17, purified immunoglobulin, buffered aqueous glycerol solution

Synonym: Anti-MBP, Monoclonal Anti-Maltose Binding Protein



Related Categories Alphabetical Index, Antibodies, Antibodies to Peptide and Protein Fusion Tags, M1-MAR, Maltose Binding Domain (MBP),
biological source   mouse
antibody form   purified immunoglobulin
clone   MBP-17, monoclonal
form   buffered aqueous glycerol solution
application(s)   western blot: 1:400 using using purified, recombinant MBP
isotype   IgG1
conjugate   alkaline phosphatase conjugate
shipped in   wet ice
storage temp.   2-8°C



Purified, recombinant MBP fusion protein

Physical form

Solution in 0.05 M Tris buffer, containing 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide


Endogenous intracellular levels of MBP in salmonella cells were determined by western blot analysis using a monoclonal anti-MBP antibody.

Monoclonal Anti-Maltose Binding Protein-Alkaline Phosphatase antibody is suitable for use in ELISA.

General description

Maltose Binding Protein (MBP) is often used to tag recombinant proteins to enhance the yield and facilitate the purification of the fusion product. Additionally, MBP tags can also improve the immunogenicity of the tagged protein. Thus, MBP can also be used as a carrier protein for vaccinations.

The antibody recognizes native as well as denatured-reduced forms of purified MBP and MBP fusion proteins.

Price and Availability

Amplified Detection
Safety & Documentation

Safety Information

GHS08  GHS08
Signal word 
Hazard statements 
Precautionary statements 
NONH for all modes of transport
WGK Germany 
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

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