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  • A4095 - Anti-phospho-ATF-2 (pThr69,71) antibody, Mouse monoclonal

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A4095 Sigma

Anti-phospho-ATF-2 (pThr69,71) antibody, Mouse monoclonal

clone ATF-22P, purified from hybridoma cell culture

Synonym: Monoclonal Anti-phospho-ATF-2 (pThr69,71)

Properties

Related Categories AA - CA, AS-ATG, Alphabetical Index, Antibodies, Antibodies for Gene Regulation,
species reactivity   rat, chicken, frog, mouse, human
application(s)   immunocytochemistry: suitable
  indirect ELISA: suitable
  microarray: suitable
  western blot: suitable
clone   ATF-22P, monoclonal
concentration   0.5-1.0 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 60-70 kDa (triplet)
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... ATF2(1386)
mouse ... Atf2(11909)
rat ... Atf2(81647)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to the N-terminal amino acids 66-79 (pT69,71) of human ATF2. This sequence is identical in ATF2 from human, rat, mouse, chicken, and frog.

General description

ATF2 (Activating Transcription Factor 2) is a member of the leucine zipper family of transcription factors. It binds to CRE domains and stimulates CRE-dependent transcription.

Mouse monoclonal anti-phospho-ATF-2 (pThr69,71) reacts specifically with ATF2 phosphorylated at both threonine 69 and 71, and does not detect the monophosphorylated and the non-phosphorylated ATF2 molecule. The epitope recognized by the antibody resides in the N-terminal region of ATF2 (aa 66-79, pT69,71). This epitope is identical in ATF2 of human, rat, mouse, chicken and frog. The antibody cross-reacts with human, rat and mouse ATF2.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Preparation Note

Purified from culture supernatant of hybridoma cells grown in a bioreactor.

Application

Mouse monoclonal anti-phospho-ATF-2 (pThr69,71) antibody can be used for immunocytochemistry, indirect ELISA, microarray, and western blot.

Price and Availability


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