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A4213 Sigma

Monoclonal Anti-Maltose Binding Protein−Peroxidase antibody produced in mouse

clone MBP-17, purified immunoglobulin, lyophilized powder

Synonym: Anti-MBP, Monoclonal Anti-Maltose Binding Protein

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies to Peptide and Protein Fusion Tags, M1-MAR, Maltose Binding Domain (MBP),
biological source   mouse
antibody form   purified immunoglobulin
clone   MBP-17, monoclonal
form   lyophilized powder
packaging   vial of 0.5 mL
application(s)   western blot: 1:1,000 using purified, recombinant MBP
isotype   IgG1
conjugate   peroxidase conjugate
storage temp.   2-8°C

Description

Immunogen

Purified, recombinant MBP fusion protein

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Reconstitution

The antibody conjugate should be reconstituted with 0.5 mL deionized water.

Physical form

Lyophilized in 0.01 M sodium phosphate buffered saline containing 1% bovine serum albumin and 0.01% merthiolate.

Application

Endogenous intracellular levels of MBP in salmonella cells were determined by western blot analysis using a monoclonal anti-MBP antibody.

General description

Maltose Binding Protein (MBP) is often used to tag recombinant proteins to enhance the yield and facilitate the purification of the fusion product. Additionally, MBP tags can also improve the immunogenicity of the tagged protein. Thus, MBP can also be used as a carrier protein for vaccinations.

The antibody recognizes native as well as denatured-reduced forms of purified MBP and MBP fusion proteins.

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
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