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A4335 Sigma

Monoclonal Anti-Myosin (Skeletal, Fast)−Alkaline Phosphatase antibody produced in mouse

clone MY-32, purified from hybridoma cell culture

Synonym: Monoclonal Anti-Myosin (Skeletal, Fast) antibody produced in mouse

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Actin-associated Proteins/Myosin, Antibodies to Cell and Organelle Proteins,
species reactivity   feline, rat, bovine, chicken, human, mouse, rabbit, guinea pig
application(s)   direct immunofluorescence: 1:150 using formalin-fixed, paraffin-embedded human or animal skeletal muscle sections
clone   MY-32, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous glycerol solution
isotype   IgG1
shipped in   wet ice
storage temp.   2-8°C
Gene Information   human ... MYH1(4619), MYH2(4620)
mouse ... Myh1(17879), Myh2(17882)
rat ... Myh1(287408), Myh2(691644)
biological source   mouse
conjugate   alkaline phosphatase conjugate

Description

Immunogen

rabbit muscle myosin.

General description

Localizes an epitope on the myosin heavy chain. Stains the fast (type II) and neonatal isomyosin molecules found in skeletal muscle, but does not stain cardiac muscle, smooth muscle or non-muscle myosin in cultured cells. Does react with human rhabdomyosarcomas.

Myosins are motor proteins that interact with actin filaments to regulate cell movement. Myosins also modulate cell shape and signaling. Skeletal muscle myosins have three ′fast′ myosin heavy chains isoforms (IIa, IIx, and IIb), a ′slow′ beta-myosin heavy chain isoform and three major myosin light-chain (MLC) isoforms. These myosin isoforms regulate skeletal muscle shortening. Monoclonal Anti-Myosin (Skeletal, Fast) specifically binds to an epitope in adult rat skeletal myosin heavy chains IIa, IIb and IId (IIx) (in fast-twitch fibers). The antibody product is also reactive in humans, bovines, cats, rabbits, mice, guinea pigs and chickens.

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol, and 15 mM sodium azide.

Application

Mouse monoclonal anti-myosin (skeletal, fast)-alkaline phosphatase antibody has been used at 1:200 and 1:400 dilutions in C2C12 cells. The antibody can also be used for direct immunofluorescence at a dilution of 1:150, using formalin-fixed, paraffin-embedded human or animal skeletal muscle sections. The antibody product may also be used for immunoenzymatic assays using frozen or formalin-fixed sections

The level of mysosin (fast) in serum samples from sportsmen with past injury was determined by western blot using monoclonal mouse anti-myosin (skeletal/fast) as the primary antibody at a dilution of 1:90000.

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