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A4403 Sigma

L-Ascorbic acid

powder, cell culture tested, γ-irradiated

Synonym: L-Threoascorbic acid, Antiscorbutic factor, Vitamin C

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Properties

Related Categories Amino Acids & Vitamins, Amino Acids and Vitamins, Biochemicals and Reagents, Cell Culture, Core Bioreagents,
InChI Key   CIWBSHSKHKDKBQ-JLAZNSOCSA-N
sterility   γ-irradiated
form   powder
color   white
pH   1.0 - 2.5 (25 °C, 176 g/L in water)
mp   190-194 °C (dec.)
solubility   H2O: soluble10 mg/mL
suitability   cell culture tested
Gene Information   human ... SLC23A2(9962)

Description

Application

For use in cell culture applications as an antioxidant and to regenerate tocopherol.
L-ascorbic acid has been used:
• in an osteogenic supplement for the growth of cells
• as a component in the differentiation media
• in WS-MSCs (Werner syndrome-mesenchymal stem cells) to study rescuing properties of ascorbic acid on aging defects

Biochem/physiol Actions

L-ascorbic acid mainly exhibits antioxidant properties. It protects plants from oxidative stress and mammals from diseases associated with oxidative stress. L-ascorbic acid mainly protects from hydroxyl radicals, superoxide and singlet oxygen. In addition, it also reduces the membrane-linked antioxidant α-tocopherol (oxidised form). L-Ascorbic acid enhances endothelium-dependent vasodilation in various disorders, including diabetes, coronary artery disease, hypertension and chronic heart failure.

General description

L-ascorbic acid is an unsaturated γ-lactone ring containing an enediol configuration (–COH=COH) attached with a carbonyl group.

Price and Availability


Laboratory Gloves

All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
CI7650000
Protocols & Articles

Articles

3D Organoid Culture: New In Vitro Models of Development and Disease

Model systems drive biological research by recapitulating body processes and functions from the molecular to whole organism level. The human body is composed of both cellular and non-cellular materia...
Keywords: Adhesion, Cell attachment, Cell culture, Diseases, Growth factors, Infectious Diseases, Processes and Functions, Vitamins

Naive Pluripotent Stem Cell Culture in an Inhibitor Based 2i/LIF Containing Serum-Free and Feeder-Free Stem Cell Media

Naive pluripotent stem cells are located within the epiblast of mature blastocysts. These primitive “ground-state” cells may be cultured in vitro using specialized media and small molecule inhibitors...
Gabi Tremml, Ming Li and Vi Chu1*,
1MilliporeSigma, Bioscience Division, Temecula, CA, USA
 
Keywords: Cell culture, Gene expression, Immunocytochemistry, Microscopy, Polymerase chain reaction, Polymerase chain reaction - quantitative, Transcription

Protocols

Induced Pluripotent Stem Cell Reprogramming Protocols

Introduction Methods     Reprogramming of Human Fibroblasts using Non-Integrating Self-Replicating RNA Vectors     Reprogramming of Peripheral Blood Mononuclear Cells (PBMCs) using STEMCCA Lentiviral...
Keywords: Apoptosis, Cell culture, Culture media, Gene expression, Growth factors, Immunocytochemistry, Polymerase chain reaction, Respiratory, Transduction, Transfection

Related Content

Ascorbate in Cell Culture

Importance and uses of ascorbate in serum-free eucaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures
Keywords: Cell culture, Culture media, Hydroxylations, Lipid peroxidation, Metabolism, Peroxidations, Vitamins

Peer-Reviewed Papers
15

References

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