A4416 Sigma

Anti-Mouse IgG (whole molecule)–Peroxidase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Properties

Related Categories Alphabetical Index, Antibodies, MO-MR, Mouse Secondary Antibodies and Conjugates, Peroxidase Labeled Antibodies,
antibody form   affinity isolated antibody
clone   polyclonal
form   buffered aqueous solution
species reactivity   mouse
application(s)   direct ELISA: 1:10,000
shipped in   dry ice
storage temp.   −20°C

Description

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin and 0.01% thimerosal.

Application

Western blot analysis of transfected Hela cell lysates was performed using HRP conjugated goat anti-mouse IgG as the secondary at a dilution of 1:4000.1

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