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  • A4416 - Anti-Mouse IgG (whole molecule)–Peroxidase antibody produced in goat

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A4416 Sigma

Anti-Mouse IgG (whole molecule)–Peroxidase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, MO-MR, Mouse Secondary Antibodies and Conjugates, Peroxidase Labeled Antibodies,
antibody form   affinity isolated antibody
clone   polyclonal
form   buffered aqueous solution
species reactivity   mouse
application(s)   direct ELISA: 1:10,000
conjugate   peroxidase conjugate
shipped in   dry ice
storage temp.   −20°C

Description

Immunogen

Purified mouse IgG

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin and 0.01% thimerosal.

Application

Anti-Mouse IgG (whole molecule)-Peroxidase antibody is suitable for use in immunoblot (1:20001,2). The product can also be used for direct ELISA: 1:10,000.

Western blot analysis of transfected Hela cell lysates was performed using HRP conjugated goat anti-mouse IgG as the secondary at a dilution of 1:4000.

General description

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases3. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice4. Anti-Mouse IgG (whole molecule)-Peroxidase antibody is specific for mouse IgGs.

Price and Availability

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Safety & Documentation

Safety Information

WGK Germany 
2

Protocols & Articles

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Keywords: Buffers, Cell biology, Epigenetics, Immunohistochemistry, Immunoprecipitation, Molecular biology, Neuroscience, Western blot

Peer-Reviewed Papers

References

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1. Cell cycle exit during terminal erythroid differentiation is associated with accumulation of (p27Kip1) and inactivation of cdk2 kinase Hsieh, F., F., et al. Blood 96(8), 2746-2754, (2000)

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2. Induction of Hepatic Transporters Multidrug Resistance-Associated Proteins (Mrp) 3 and 4 by Clofibrate Is Regulated by Peroxisome Proliferator-Activated Receptor α Moffit, J.S., et al. J. Pharmacol. Exp. Ther. 317(2), 537-545, (2006)

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3. Structure and Function of Human and Murine Receptors for IgG. Unkeless, J.C., et al. Annu. Rev. Immunol. 6, 251-281, (1988)

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4. Complement-fixing IgG1 constitutes a new subclass of mouse IgG Peter, L. EY, et al. Nature 281, 492 - 493, (1979)

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ABCG2 Is Upregulated In Alzheimer's Brain With Cerebral Amyloid Angiopathy And May Act As A Gatekeeper At The Blood-brain Barrier For Abeta(1-40) Peptides. Xiong, H., et al. J. Neurosci. 29, 5463 - 5475, (2009)

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Hepatocyte Nuclear Factor 4α Contributes to Thyroid Hormone Homeostasis by Cooperatively Regulating the Type 1 Iodothyronine Deiodinase Gene with GATA4 and Krüppel-Like Transcription Factor 9 Uchida, A., et al. Mol. Cell. Biol. 28, 3917-3931, (2008)

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SCF(FBW7) regulates cellular apoptosis by targeting MCL1 for ubiquitylation and destruction. Inuzuka, H., et al. Nature 471, 104-9, (2011)

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Synthetic ion transporters can induce apoptosis by facilitating chloride anion transport into cells. Ko SK, Kim SK, Share A, et al. Nature Chemistry 6(10), 885-92, (2014)

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Assembly and architecture of precursor nodes during fission yeast cytokinesis. Laporte, D., et al. J. Cell Biol. 192, 1005-21, (2011)

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The relationship between human skeletal muscle pyruvate dehydrogenase phosphatase activity and muscle aerobic capacity. Love, L.K., et al. J. Appl. Physiol. 111, 427-34, (2011)

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Tissue distribution and gender-divergent expression of 78 cytochrome P450 mRNAs in mice. Renaud, H.J., et al. Toxicol. Sci. 124, 261-77, (2011)

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Antagonistic effects of cellular poly(C) binding proteins on vesicular stomatitis virus gene expression. Dinh, P.X., et al. J. Virol. 85, 9459-71, (2011)

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Phenylephrine preconditioning involves modulation of cardiac sarcolemmal K(ATP) current by PKC delta, AMPK and p38 MAPK. Turrell, H.E., et al. J. Mol. Cell. Cardiol. 51, 370-80, (2011)

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Assays for studying nucleated aggregation of polyglutamine proteins. Jayaraman, M., et al. Methods 53, 246-54, (2011)

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Detection of botulinum neurotoxin serotype B at sub mouse LD(50) levels by a sandwich immunoassay and its application to toxin detection in milk. Scotcher, M.C., et al. PLoS ONE 5, e11047, (2010)

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Functional interaction between dynein light chain and intermediate chain is required for mitotic spindle positioning. Stuchell-Brereton, M.D., et al. Mol. Biol. Cell 22(15), 2690-2701, (2011)

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Hepatitis C virus core protein induces malignant transformation of biliary epithelial cells by activating nuclear factor-kappaB pathway. Li, Z.H., et al. J. Gastroenterol. Hepatol. 25, 1315-20, (2010)

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Induction Of Neutralizing Antibody Responses To Anthrax PA Using Influenza Vectors: Implications For Disparate Immune System Priming Pathways Langley, W., et al. J. Virol. 84, 8300-7, (2010)

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Evaluation of the protective potential of a Taenia solium cysticercus mimotope on murine cysticercosis. Capelli-Peixoto, J., et al. Vaccine 29, 9473-9, (2011)

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In vitro-Ishikawa cell test for assessing tissue-specific chemical effects on human endometrium. Schaefer, W.R., et al. Reprod. Toxicol. 30, 89-93, (2010)

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Brain-specific Deletion Of Extracellular Signal-regulated Kinase 2 Mitogen-activated Protein Kinase Leads To Aberrant Cortical Collagen Deposition. Heffron, D.S., et al. Am. J. Pathol. 175, 2586-99, (2009)

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Epitope Characterization And Variable Region Sequence Of F1-40, A High-affinity Monoclonal Antibody To Botulinum Neurotoxin Type A (Hall Strain). Scotcher, M.C., et al. PLoS ONE 4, e4924, (2009)

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The induction of a nucleoplasmic reticulum by prelamin A accumulation requires CTP:phosphocholine cytidylyltransferase-α. Goulbourne, C.N., et al. J. Cell Sci. 124, 4253-66, (2011)

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BRCA1 negatively regulates formation of autophagic vacuoles in MCF-7 breast cancer cells. Esteve, J.M., et al. Exp. Cell Res. 316, 2618-29, (2010)

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The Interaction of Herpes Simplex Virus 1 Regulatory Protein ICP22 with the cdc25C Phosphatase Is Enabled in vitro by Viral Protein Kinases US3 and UL13 Roizman, B., et al. J. Virol. 82, 4533-4543, (2008)

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Functional interactions of Rec24, the fission yeast ortholog of mouse Mei4, with the meiotic recombination-initiation complex. Bonfils, S., et al. J. Cell Sci. 124, 1328-38, (2011)

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Mycoplasma gallisepticum and Mycoplasma synoviae express a cysteine protease CysP, which can cleave chicken IgG into Fab and Fc. Cizelj, I., et al. Microbiology 157, 362-72, (2011)

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Effect of HFE variants on sphingolipid expression by SH-SY5Y human neuroblastoma cells. Ali-Rahmani, F., et al. Neurochem. Res. 36, 1687-96, (2011)

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Baculovirus production of fully-active phosphoinositide 3-kinase alpha as a p85alpha-p110alpha fusion for X-ray crystallographic analysis with ATP competitive enzyme inhibitors. Sinnamon, R.H., et al. Protein Expr. Purif. 73, 167-76, (2010)

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Evidence that core histone H3 is targeted to the mitochondria in Brassica oleracea. Katherine, B., et al. Cell Biol. Int. 34, 997-1003, (2010)

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Blau syndrome polymorphisms in NOD2 identify nucleotide hydrolysis and helical domain 1 as signalling regulators. Parkhouse R, Boyle JP, and Monie TP FEBS Lett. 588(18), 3382-9, (2014)

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Immune responses elicited against rotavirus middle layer protein VP6 inhibit viral replication in vitro and in vivo. Lappalainen S, Pastor AR, Tamminen K, et al. Hum. Vaccin. Immunother. 10(7), 2039-47, (2014)

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Negative regulation of DAB2IP by Akt and SCFFbw7 pathways. Dai X, North BJ, and Inuzuka H Oncotarget 5(10), 3307-15, (2014)

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The COP II adaptor protein TMED7 is required to initiate and mediate the delivery of TLR4 to the plasma membrane. Liaunardy-Jopeace A, Bryant CE, and Gay NJ Sci. Signal. 7(336), ra70, (2014)

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Evaluation of apoptogenic adenovirus type 5 oncolytic vectors in a Syrian hamster head and neck cancer model. Vijayalingam S, Kuppuswamy M, Subramanian T, et al. Cancer Gene Ther. 21(6), 228-37, (2014)

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