Technical information & documentation associated with this product is available in the
Safety & Documentation tab.
Protocols
Enzymatic Assay of Hyaluronidase
1. OBJECTIVE To standardize a procedure for determining the activity of Hyaluronidase at Sigma-Aldrich St. Louis.
Keywords: Extinction coefficient
Enzymatic Assay of L-Lactic Dehydrogenase (EC 1.1.1.27)
1. OBJECTIVE To standardize a procedure for the assay of L-Lactic Dehydrogenase for all sources from heart muscle.
Keywords: Extinction coefficient
Enzymatic Assay of Lipase Type XIII from Pseudomonas Species and Pseudomonas Cepacia using a coupled enzyme system of Glycerol Kinase and Glycerophosphate Oxidase (EC 3.1.1.3)
1. OBJECTIVE To standardize a procedure for the enzymatic assay of Lipase Type XIII from Pseudomonas Species, Sigma Product Number L9518 , and Pseudomonas Cepacia ,Sigma Product Number L9156, using a...
Keywords: Centrifugation, Extinction coefficient, High performance liquid chromatography
Enzymatic Assay of Myokinase (EC 2.7.4.3)
Replaces OP SP-ADP01. New document assigned to conform to current document numbering system. Refer to CR SOP-DEK-ENZ47.
Keywords: Extinction coefficient, Molecular biology
Enzymatic Assay of Nuclease S1, Product No. N5661
1. OBJECTIVE To standardize a procedure for the enzymatic assay of Nuclease S1, Sigma Product Number N5661 at Sigma-Aldrich St. Louis.
Enzymatic Assay of Peroxidase (EC 1.11.1.7) 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a Substrate
Replaces SPABTS02. Procedure updated to conform to current QUMAS formatting guidelines. Refer to CR SOP-DEK-ENZ42.
Keywords: Extinction coefficient
Enzymatic Assay of β-Glucuronidase (EC 3.2.1.31) from E. coli
2. SCOPE The scope of this procedure is all products from E. coli that have a specification for β-Glucuronidase activity.
Keywords: Enzymology
Papers
Set your institution to view full text papers.
1. Characterization of bovine serum albumin epitopes and their role in allergic reactions Restani, P., et al. Allergy 59(Suppl. 78), 21-24, (2004)
Loading...
Preparation and properties of serum and plasma proteins; a system for the separation into fractions of the protein and lipoprotein components of biological tissues and fluids. Cohn, E.J., et al. J. Am. Chem. Soc. 68, 459, (1946)
Loading...
Mechanisms of confluence-dependent expression of CD26 in colon cancer cell lines. Abe, M., et al. BMC Cancer 11, 51, (2011)
Loading...
H5N1 avian influenza virus induces apoptotic cell death in mammalian airway epithelial cells. Tomo Daidoji et al J. Virol. 82, 11294-307, (2008)
Loading...
Contact-dependent growth inhibition requires the essential outer membrane protein BamA (YaeT) as the receptor and the inner membrane transport protein AcrB. Stephanie K. Aoki et al Mol. Microbiol. 70, 323-40, (2008)
Loading...
Fly transmission and mating of Trypanosoma brucei brucei strain 427. Lori Peacock et al Mol. Biochem. Parasitol. 160, 100-6, (2008)
Loading...
Phosphorylation-independent regulation of the diguanylate cyclase WspR. Nabanita De et al PLoS Biol. 6, e67, (2008)
Loading...
Red Cabbage (Brassica oleracea) Ameliorates Diabetic Nephropathy in Rats. Hazem A. H. Kataya et al Evid. Based. Complement. Alternat. Med. 5, 281-7, (2008)
Loading...
Phase I study of a herpes simplex virus type 2 (HSV-2) DNA vaccine administered to healthy, HSV-2-seronegative adults by a needle-free injection system. Ashok Cattamanchi et al Clin. Vaccine Immunol. 15, 1638-43, (2008)
Loading...
Diversity in genomic organisation, developmental regulation and distribution of the murine PR72/B" subunits of protein phosphatase 2A. Karen Zwaenepoel et al BMC Genomics 9, 393, (2008)
Loading...
Autocrine activation of neuronal NMDA receptors by aspartate mediates dopamine- and cAMP-induced CREB-dependent gene transcription. Luis E. F. Almeida et al J. Neurosci. 29, 12702-10, (2009)
Loading...
The pseudomonas toxin pyocyanin inhibits the dual oxidase-based antimicrobial system as it imposes oxidative stress on airway epithelial cells Rada B, et al. J. Immunol. 181, 4883-4893, (2008)
Loading...
Protective effects of mildronate in an experimental model of type 2 diabetes in Goto-Kakizaki rats. Edgars Liepinsh et al Br. J. Pharmacol. 157, 1549-56, (2009)
Loading...
Expression of activated STAT5 in neoplastic mast cells in systemic mastocytosis: subcellular distribution and role of the transforming oncoprotein KIT D816V. Christian Baumgartner et al Am. J. Pathol. 175, 2416-29, (2009)
Loading...
Multiple functional domains of Enterococcus faecalis aggregation substance Asc10 contribute to endocarditis virulence. Olivia N. Chuang et al Infect. Immun. 77, 539-48, (2009)
Loading...
Combination therapy in treatment of experimental pulmonary aspergillosis: in vitro and in vivo correlations of the concentration- and dose- dependent interactions between anidulafungin and voriconazole by Bliss independence drug interaction analysis. Vidmantas Petraitis et al Antimicrob. Agents Chemother. 53, 2382-91, (2009)
Loading...
A protocol for the production of recombinant spider silk-like proteins for artificial fiber spinning. Florence Teulé et al Nat. Protoc. 4, 341-55, (2009)
Loading...
Downregulation of Kv4.2 channels mediated by NR2B-containing NMDA receptors in cultured hippocampal neurons. Zhigang Lei et al Neuroscience 165, 350-62, (2010)
Loading...
Proteomic characterization of plasma membrane-proximal T cell activation responses. Ben de Wet et al J. Biol. Chem. 286, 4072-80, (2011)
Loading...
Small compound 6-O-angeloylplenolin induces mitotic arrest and exhibits therapeutic potentials in multiple myeloma. Ying Liu et al PLoS ONE 6, e21930, (2011)
Loading...
CCL28 Induces Mucosal Homing of HIV-1-Specific IgA-Secreting Plasma Cells in Mice Immunized with HIV-1 Virus-Like Particles. Veronica Rainone et al PLoS ONE 6, e26979, (2011)
Loading...
Design and analysis of rhesus cytomegalovirus IL-10 mutants as a model for novel vaccines against human cytomegalovirus. Naomi J. Logsdon et al PLoS ONE 6, e28127, (2011)
Loading...
The dynactin complex enhances the speed of microtubule-dependent motions of adenovirus both towards and away from the nucleus. Martin F. Engelke et al Viruses 3, 233-53, (2011)
Loading...
Axonal regeneration and neuronal function are preserved in motor neurons lacking ß-actin in vivo. Cheever TR PLoS ONE 6(3), e17768, (2011)
Loading...
Optimizing human synovial fluid preparation for two-dimensional gel electrophoresis. Chen CP Proteome Sci. 9, 65, (2011)
Loading...
Detection of human cytomegalovirus in motile spermatozoa and spermatogenic cells in testis organotypic culture. Naumenko VA Herpesviridae 2(1), 7, (2011)
Loading...
Super-resolution dynamic imaging of dendritic spines using a low-affinity photoconvertible actin probe. Izeddin I PLoS ONE 6(1), e15611, (2011)
Loading...
Selenium nanoparticles inhibit Staphylococcus aureus growth. Tran PA Int. J. Nanomedicine 6, 1553-8, (2011)
Loading...
Astrocyte-derived tissue transglutaminase interacts with fibronectin: a role in astrocyte adhesion and migration? van Strien ME PLoS ONE 6(9), e25037, (2011)
Loading...
Stimulation of neoplastic mouse lung cell proliferation by alveolar macrophage-derived, insulin-like growth factor-1 can be blocked by inhibiting MEK and PI3K activation. Fritz JM Mol. Cancer 10, 76, (2011)
Loading...
Evidence of fibrinogen as a target of citrullination in IgM rheumatoid factor-positive polyarticular juvenile idiopathic arthritis. Gilliam BE Pediatr. Rheumatol. Online J. 9, 8, (2011)
Loading...
Ionic components of electric current at rat corneal wounds. Vieira AC PLoS ONE 6(2), e17411, (2011)
Loading...
Transport activity of the sodium bicarbonate cotransporter NBCe1 is enhanced by different isoforms of carbonic anhydrase. Schueler C PLoS ONE 6(11), e27167, (2011)
Loading...
Site directed biotinylation of filamentous phage structural proteins. Smelyanski L Virol. J. 8, 495, (2011)
Loading...
ZEB1 limits adenoviral infectability by transcriptionally repressing the coxsackie virus and adenovirus receptor. Lacher MD Mol. Cancer 10, 91, (2011)
Loading...
Attenuation of acridine mutagen ICR-191--DNA interactions and DNA damage by the mutagen interceptor chlorophyllin. Pietrzak M Biophys. Chem. 135(1-3), 69-75, (2008)
Loading...
In vitro Growth and Characterization of Stem Cells from Human Dental Pulp of Deciduous Versus Permanent Teeth. Eslaminejad MB J. Dent. (Tehran.) 7(4), 185-95, (2010)
Loading...
Altered states of telomere deprotection and the two-stage mechanism of replicative aging. Zou Y Mol. Cell. Biol. 29(9), 2390-7, (2009)
Loading...
Insoluble, speckled cytosolic distribution of retinoic acid receptor alpha protein as a marker of hepatic stellate cell activation in vitro. Mezaki Y J. Histochem. Cytochem. 57(7), 687-99, (2009)
Loading...
Structural basis for c-di-GMP-mediated inside-out signaling controlling periplasmic proteolysis. Navarro MV PLoS Biol. 9(2), e1000588, (2011)
Loading...
Zinc oxide nanoparticles as selective killers of proliferating cells. Taccola L Int. J. Nanomedicine 6, 1129-40, (2011)
Loading...
Cell cycle control and DNA damage response of conditionally immortalized urothelial cells. Dixon BP PLoS ONE 6(1), e16595, (2011)
Loading...
S100 calgranulin proteins S100A8, S100A9 and S100A12 are expressed in the inflamed gastric mucosa of Helicobacter pylori-infected children. Leach ST Can. J. Gastroenterol. 22(5), 461-4, (2008)
Loading...
Soluble factors from neocortical astrocytes enhance neuronal differentiation of neural progenitor cells from adult rat hippocampus on micropatterned polymer substrates. Oh J J. Biomed. Mater. Res. A 91(2), 575-85, (2009)
Loading...
Glycosylation of mucins present in gastric juice: the effect of helicobacter pylori eradication treatment. Radziejewska I Clin. Exp. Med. 11(2), 81-8, (2011)
Loading...
Bacteriophages with potential for inactivation of fish pathogenic bacteria: survival, host specificity and effect on bacterial community structure. Pereira C Mar. Drugs 9(11), 2236-55, (2011)
Loading...
Aberrant anaplastic lymphoma kinase activity induces a p53 and Rb-dependent senescence-like arrest in the absence of detectable p53 stabilization. McDuff FK PLoS ONE 6(3), e17854, (2011)
Loading...
Orthotopic transplantation of immortalized mesencephalic progenitors (CSM14.1 cells) into the substantia nigra of hemiparkinsonian rats induces neuronal differentiation and motoric improvement. Haas SJ J. Anat. 212(1), 19-30, (2008)
Loading...
Mercury vapor release from broken compact fluorescent lamps and in situ capture by new nanomaterial sorbents. Johnson NC Environ. Sci. Technol. 42(15), 5772-8, (2008)
Loading...
Separate basolateral and apical phosphatidylcholine secretion routes in intestinally differentiated tumor cells. Gotthardt D World J. Gastroenterol. 15(46), 5821-6, (2009)
Loading...
Modulation of interleukin-1β-induced inflammatory responses by a synthetic cationic innate defence regulator peptide, IDR-1002, in synovial fibroblasts. Turner-Brannen E Arthritis. Res. Ther. 13(4), R129, (2011)
Loading...
Administration of probiotics influences F4 (K88)-positive enterotoxigenic Escherichia coli attachment and intestinal cytokine expression in weaned pigs. Daudelin JF Vet. Res. 42(1), 69, (2011)
Loading...
Characterization of the expression of cytokeratins 5, 8, and 14 in mouse thymic epithelial cells during thymus regeneration following acute thymic involution. Lee EN Anat. Cell Biol. 44(1), 14-24, (2011)
Loading...
Fused eco29kIR- and M genes coding for a fully functional hybrid polypeptide as a model of molecular evolution of restriction-modification systems. Mokrishcheva ML BMC Evol. Biol. 11, 35, (2011)
Loading...
Role of protein kinase C and epidermal growth factor receptor signalling in growth stimulation by neurotensin in colon carcinoma cells. Müller KM BMC Cancer 11, 421, (2011)
Loading...
Resolving phenylalanine metabolism sheds light on natural synthesis of penicillin G in Penicillium chrysogenum. Veiga T Eukaryotic Cell 11(2), 238-49, (2012)
Loading...
Prognostic significance of heat shock protein 70 (HSP70) in patients with oral cancer. Tavassol F Head Neck Oncol. 3, 10, (2011)
Loading...
Peroxisome proliferator-activated receptor-gamma agonists suppress tissue factor overexpression in rat balloon injury model with paclitaxel infusion. Park JB PLoS ONE 6(11), e28327, (2011)
Loading...
Striking denervation of neuromuscular junctions without lumbar motoneuron loss in geriatric mouse muscle. Chai RJ PLoS ONE 6(12), e28090, (2011)
Loading...
The role of sialyl glycan recognition in host tissue tropism of the avian parasite Eimeria tenella. Lai L PLoS Pathog. 7(10), e1002296, (2011)
Loading...
In vivo stable tumor-specific painting in various colors using dehalogenase-based protein-tag fluorescent ligands. Kosaka N Bioconjug. Chem. 20(7), 1367-74, (2009)
Loading...
Mouse early oocytes are transiently polar: three-dimensional and ultrastructural analysis. Kloc M Exp. Cell Res. 314(17), 3245-54, (2008)
Loading...
Genetic diversity of heat-labile toxin expressed by enterotoxigenic Escherichia coli strains isolated from humans. Lasaro MA J. Bacteriol. 190(7), 2400-10, (2008)
Loading...
Adiponectin-coated nanoparticles for enhanced imaging of atherosclerotic plaques. Almer G Int. J. Nanomedicine 6, 1279-90, (2011)
Loading...
Lentiviral-mediated genetic correction of hematopoietic and mesenchymal progenitor cells from Fanconi anemia patients. Jacome A Mol. Ther. 17(6), 1083-92, (2009)
Loading...
Identification of a novel self-sufficient styrene monooxygenase from Rhodococcus opacus 1CP. Tischler D J. Bacteriol. 191(15), 4996-5009, (2009)
Loading...
Phosphatase inhibitors with anti-angiogenic effect in vitro. Sylvest L APMIS 118(1), 49-59, (2010)
Loading...
Preparation and properties of serum and plasma proteins; a system for the separation into fractions of the protein and lipoprotein components of biological tissues and fluids. Cohn, E.J., et al. J. Am. Chem. Soc. 68, 459, (1946)
Loading...
Merck 14,8468