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A4503 Sigma

Bovine Serum Albumin

cold ethanol fraction, pH 5.2, ≥96%

Synonym: Albumin bovine serum, BSA, Bovine albumin

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Packaging

1, 5 kg in poly drum

10, 50, 100, 500 g in poly bottle

Preparation Note

Often referred to as Cohn fraction V; this product is prepared by a modified method of the Cohn cold ethanol fractionation method.

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

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Safety & Documentation
Protocols & Articles

Protocols

Enzymatic Assay of Hyaluronidase (3.2.1.35)

The turbidimetric determination (% Transmittance at 600 nm, Light path = 1 cm) is based on the following reaction:
Keywords: Extinction coefficient

Enzymatic Assay of L-Lactic Dehydrogenase (EC 1.1.1.27)

To standardize a procedure for the assay of L-Lactic Dehydrogenase for all sources from heart muscle.
Keywords: Extinction coefficient

Enzymatic Assay of Lipase Type XIII from Pseudomonas Species and Pseudomonas Cepacia using a coupled enzyme system of Glycerol Kinase and Glycerophosphate Oxidase (EC 3.1.1.3)

To standardize a procedure for the enzymatic assay of Lipase Type XIII from Pseudomonas Species, Sigma Product Number L9518 , and Pseudomonas Cepacia, Sigma Product Number L9156, using a coupled enzy...
Keywords: Centrifugation, Extinction coefficient, High performance liquid chromatography

Enzymatic Assay of Myokinase (EC 2.7.4.3)

Replaces OP SP-ADP01. New document assigned to conform to current document numbering system. Refer to CR SOP-DEK-ENZ47.
Keywords: Extinction coefficient, Molecular biology

Enzymatic Assay of Nuclease S1, Product No. N5661

To standardize a procedure for the enzymatic assay of Nuclease S1, Sigma Product Number N5661 at Sigma-Aldrich St. Louis.

Enzymatic Assay of Peroxidase (EC 1.11.1.7) 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a Substrate

Replaces SPABTS02. Procedure updated to conform to current QUMAS formatting guidelines. Refer to CR SOP-DEK-ENZ42.
Keywords: Extinction coefficient

Enzymatic Assay of β-Glucuronidase (EC 3.2.1.31) from E. coli

The scope of this procedure is all products from E. coli that have a specification for β-Glucuronidase activity.
Keywords: Enzymology

Peer-Reviewed Papers
15

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