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A4529 Sigma

Aprotinin from bovine lung

lyophilized powder, 3-7 TIU/mg solid

Synonym: Trasylol, Trypsin inhibitor (basic)

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Properties

Related Categories Biochemicals and Reagents, Enzyme Inhibitors, Enzyme Inhibitors by Type, Enzymes, Inhibitors, and Substrates, Individual Protease Inhibitors,
Quality Level   PREMIUM
form   lyophilized powder
mol wt   mol wt ~6,500
solubility   H2O: >10 mg/mL
storage temp.   2-8°C
Gene Information   cow ... PTI(404172)

Description

Application

Aprotinin is largely used as an inhibitor of trypsin.

Packaging

1, 5 mg in glass bottle

10, 25 mg in poly bottle

100 mg in glass bottle

Preparation Note

This product is an affinity purified lyophilized powder purified to remove trace impurities. It has an activity of 3-7 TIU/mg.

Unit Definition

One Trypsin Inhibitor Unit (TIU) will decrease the activity of two trypsin units by 50%, where one trypsin unit will hydrolyze 1.0 μmole of N-alpha-benzoyl-DL-arginine p-nitroanilide per minute at pH 7.8 and 25°C. Another commonly used unit is the KIU, with 1 TIU = 1,300 KIU.

Biochem/physiol Actions

Aprotinin is a competitive serine protease inhibitor that forms stable complexes with and blocks the active sites of enzyme. This binding is reversible, and most aprotinin-protease complexes will dissociate at extreme pH levels >10 or <3. Structurally, Aprotinin is a monomeric globular protein derived from bovine lung that consists of 58 amino acids, arranged in a single polypeptide chain with three crosslinking disulfide bridges.

Price and Availability


Ultrafree Centrifugal Filter Units

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
YN5080000
Protocols & Articles

Articles

HPLC Analysis of Proteins on SRT® SEC-1000, 30 cm x 7.8 mm I.D., 5 μm particles

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on SRT® SEC-1000, 30 cm x 7.8 mm I.D., 5 μm particles
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Proteins on SRT® SEC-300, 30 cm x 7.8 mm I.D., 5 μm particles

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on SRT® SEC-300, 30 cm x 7.8 mm I.D., 5 μm particles
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

Protocols

Enzymatic Assay of Aprotinin

The scope of this procedure is all products that have a specification for Aprotinin activity.
Keywords: Enzymology, Extinction coefficient

Peer-Reviewed Papers
15

References

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