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A4540 Sigma

Anti-Mouse IgM (μ-chain specific)−Agarose antibody produced in goat

IgG fraction of antiserum, saline suspension

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Properties

Related Categories Affinity Chromatography, Agarose, Alphabetical Index, Antibodies, Antibody Purification and Characterization,
biological source   goat
antibody form   IgG fraction of antiserum
clone   polyclonal
form   saline suspension
species reactivity   mouse
extent of labeling   ≥5 mg per mL
application(s)   Ouchterlony double diffusion: suitable
capacity   0.4 mg binding capacity
conjugate   agarose conjugate
storage temp.   2-8°C

Description

Physical form

Suspension in 0.5 M NaCl and 0.01% thimerosal.

Application

Goat anti-mouse IgM (μ-chain specific)-agarose antibody has been used for immunoprecipitation and ChIP assays. The product can also be used for Ouchterlony double diffusion applications.

To isolate chimeric proteins that were fused with the IgM heavy chain, CHO supernatant was ran over a column packed with agarose-linked goat anti-mouse IgM mu chain specific.

General description

IgM is a glycoprotein antibody that regulates humoral immune responses. Mouse IgM is involved in modulating B cell memory. This antibody isotype has also been implicated in the development of autoimmune responses associated with the pathogenesis of type 1 diabetes in mice.
Goat anti-mouse IgM (μ-chain specific)-agarose antibody is specific for mouse IgM when tested against purified mouse IgA, IgG (all subclasses), and IgM myeloma proteins.

Price and Availability


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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

Protocols

Enzymatic Assay of Protease, Product No. P2922

To standardize a procedure for the enzymatic assay of Protease activity with N-t-Boc-L-glutamic acid α-phenyl ester substrate.
Keywords: Digestions, Enzymology, Extinction coefficient

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Peer-Reviewed Papers
15

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