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A4541 Sigma

Anti-Biotin−Peroxidase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-Biotin

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies to Biotin, B-BI, Biotin and Biotin Antibodies,
biological source   goat
antibody form   affinity isolated antibody
clone   polyclonal
form   buffered aqueous solution
application(s)   direct ELISA: 1:2,000
conjugate   peroxidase conjugate
shipped in   dry ice
storage temp.   −20°C

Description

Immunogen

Biotin-KLH conjugate

Packaging

0.25, 0.5 mL in glass insert

1 mL in glass bottle

Preparation Note

Prepared by the periodate method described by Wilson, M.B., and Nakane, P.K., in "Immunofluorescence and Related Staining Techniques," Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.01% thimerosal

Application

Biotin-labeled proteins in U937 and BHK cell lysates were analyzed by western blot using HRP-conjugated goat anti-biotin.

Goat anti-biotin-peroxidase antibody can be used for direct ELISA assays at a dilution of 1:2,000.

In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.

General description

Biotin is an essential vitamin required for cells in living organisms and in cultures. The high binding affinity of biotin to egg white avidin or bacteria-derived streptavidin has been used to design immunoassays for detecting antigen-antibody interactions. Goat anti-biotin-peroxidase antibody can be used in many applications where biotin may be introduced as target label.

Specificity and reactivity are determined by Ouchterlony Double Diffusion and immunoelectrophoresis versus the biotin-carrier protein complex. No reactivity is observed versus the carrier protein.

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Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
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