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A4700 Sigma

Monoclonal Anti-Actin antibody produced in mouse

clone AC-40, ascites fluid

Synonym: Monoclonal Anti-Actin

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Properties

Related Categories AC-AC, Actin Antibodies, Alphabetical Index, Antibodies, Antibodies for Cell Biology,
species reactivity   goat, viper, Xenopus, bovine, guinea pig, snail, canine, hamster, rat, chicken, mouse, human, sheep, pig, rabbit, carp
application(s)   immunohistochemistry (frozen sections): suitable using methanol-fixed, frozen sections
  indirect ELISA: suitable
  indirect immunofluorescence: 1:200 using cultured human or chicken fibroblasts
  western blot: 1:500 using cultured human or chicken fibroblast extract
clone   AC-40, monoclonal
antibody form   ascites fluid
isotype   IgG2a
mol wt   antigen mol wt 42 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
biological source   mouse
conjugate   unconjugated

Description

Immunogen

synthetic actin C-terminal peptide Ser-Gly-Pro-Ser-Ile-Val-His-Arg-Lys-Cys-Phe, attached to a Multiple Antigen Peptide (MAP) backbone.

General description

The antibody recognizes an epitope located on the C-terminal end of actin conserved in all actin isoforms. It specifically labels actin in a wide variety of tissues and species. The epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding. Zinc-formalin, B5, ethanol, methacarn, Brunnel′s or Bouin′s solutions may also be used as fixatives.

The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in cellular process including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms characterized by electrophoresis and amino acid sequence analysis.
Four of them represent the differentiation markers of muscle tissues and two are found practically in all cells. There are three a-actins (skeletal, cardiac, and smooth muscle), one b-actin (b-nonmuscle), and two g-actins (g-smooth muscle and g-nonmuscle). Actin isoforms show >90% overall sequence homology, but only 50–60% homology in their NH2-terminal residues.

Physical form

Supplied as ascites fluid with 15mM sodium azide as a preservative.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
• Chromatin immunoprecipitation (1 paper)
• Immunohistochemistry (1 paper)
• Western Blotting (1 paper)
• Western blotting following immunoprecipitation (1 paper)

Mouse monoclonal anti-actin antibody can be used for western blotting applications. The antibody can also be used for IHC using frozen, methanol-fixed sections. Furthermore, the product can be used for indirect immunofluorescence (1:200) and indirect ELISA.

Monoclonal anti-actin antibody was used for western blot analysis of Cos-7 cell lysates to ensure equal protein loading.

Western blot analysis of MDCK cell lysates were performed using monoclonal anti-actin antibody as a primary antibody.

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

Articles

Antibodies to Actin Gene Family Isoforms

The Actin Gene Family Actin and myosin are the two major cytoskeletal proteins implicated in cell motility. As constituents of many cell types, actin and myosin are involved in a myriad of cellular p...
Keywords: Western blot

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Peer-Reviewed Papers
15

References

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06693 Timestrip Plus -20 °C

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