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A4720 Sigma

Monoclonal Anti-Phosphotyrosine−Agarose antibody produced in mouse

clone PY20, purified immunoglobulin, buffered aqueous suspension

Synonym: Monoclonal Anti-Phosphotyrosine, Phospho-Tyr, Phospho-tyrosine, p-Tyr

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Properties

Related Categories Affinity Chromatography, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology,
biological source   mouse
antibody form   purified immunoglobulin
clone   PY20, monoclonal
form   buffered aqueous suspension
application(s)   immunoprecipitation: suitable
isotype   IgG2b
conjugate   agarose conjugate
shipped in   wet ice
storage temp.   2-8°C

Description

Immunogen

phosphotyrosine-protein conjugate

Physical form

Suspension in 20 mM sodium phosphate, pH 7.5, 150 mM sodium chloride, 3 mM sodium azide.

Application

Monoclonal anti-phosphotyrosine−agarose antibody can be used in immunoaffinity purification of phosphotyrosyl proteins, including transforming proteins, steroid hormone receptors (e.g., estradiol and glucocorticoid receptors), growth factor receptors (e.g., EGF and PDGF receptors), insulin receptors and other proteins involved in the control of cellular growth.

General description

Reversible phosphorylation of proteins is an important post-translational modification that plays a regulatory role in the expression of most proteins in the cells. Reversible phosphorylation at multiple serine, tyrosine and threonine residues mediates numerous signalling pathways in both prokaryotic and eukaryotic cells . Cellular proteins with phosphorylated tyrosine increase many fold by the activation of tyrosine kinases. Most mitogenic receptor systems such as EGF, PDGF, insulin receptors contain serine/threonine/tyrosine kinase domains that undergo autophosphorylation when receptors bind to the respective ligands. Monoclonal anti-phosphotyrosine−agarose antibody can be used in immunoprecipitation. Mouse anti-phosphotyrosine−agarose antibody reacts specifically with both native and denatured proteins comprising phosphorylated tyrosine.

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Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
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