• USA Home
  • A4720 - Monoclonal Anti-Phosphotyrosine−Agarose antibody produced in mouse

A4720 Sigma

Monoclonal Anti-Phosphotyrosine−Agarose antibody produced in mouse

clone PY20, purified immunoglobulin, buffered aqueous suspension

Synonym: Monoclonal Anti-Phosphotyrosine, Phospho-Tyr, Phospho-tyrosine, p-Tyr



Related Categories Affinity Chromatography, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology,
biological source   mouse
antibody form   purified immunoglobulin
clone   PY20, monoclonal
form   buffered aqueous suspension
application(s)   immunoprecipitation: suitable
isotype   IgG2b
conjugate   agarose conjugate
shipped in   wet ice
storage temp.   2-8°C



phosphotyrosine-protein conjugate

Physical form

Suspension in 20 mM sodium phosphate, pH 7.5, 150 mM sodium chloride, 3 mM sodium azide.


Monoclonal anti-phosphotyrosine−agarose antibody can be used in immunoaffinity purification of phosphotyrosyl proteins, including transforming proteins, steroid hormone receptors (e.g., estradiol and glucocorticoid receptors), growth factor receptors (e.g., EGF and PDGF receptors), insulin receptors and other proteins involved in the control of cellular growth.

General description

Reversible phosphorylation of proteins is an important post-translational modification that plays a regulatory role in the expression of most proteins in the cells. Reversible phosphorylation at multiple serine, tyrosine and threonine residues mediates numerous signalling pathways in both prokaryotic and eukaryotic cells . Cellular proteins with phosphorylated tyrosine increase many fold by the activation of tyrosine kinases. Most mitogenic receptor systems such as EGF, PDGF, insulin receptors contain serine/threonine/tyrosine kinase domains that undergo autophosphorylation when receptors bind to the respective ligands. Monoclonal anti-phosphotyrosine−agarose antibody can be used in immunoprecipitation. Mouse anti-phosphotyrosine−agarose antibody reacts specifically with both native and denatured proteins comprising phosphorylated tyrosine.

Price and Availability

Antibody Explorer

Explore with Confidence

Amplified Detection
Safety & Documentation

Safety Information

GHS07  GHS07
Signal word 
Hazard statements 
Precautionary statements 
NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?