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  • A4721 - Monoclonal Anti-ARNO (Cytohesin-2) antibody produced in mouse

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A4721 Sigma

Monoclonal Anti-ARNO (Cytohesin-2) antibody produced in mouse

~2 mg/mL, clone CYT2-21, purified immunoglobulin, buffered aqueous solution

Properties

Related Categories AR-AR, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to ARF and Related Proteins,
species reactivity   rat, human
application(s)   immunocytochemistry: suitable
  indirect ELISA: suitable
  microarray: suitable
  western blot: 5-10 μg/mL using a whole extract of rat brain
clone   CYT2-21, monoclonal
concentration   ~2 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 40 kDa
storage temp.   −20°C
Gene Information   human ... CYTH2(9266)
rat ... Cyth2(116692)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

recombinant, human ARNO (ARFnucleotide-binding-site opener).

General description

ARNO (Cytohesin-2) is a guanine-nucleotide exchange factor that belongs to Arf family of GTP-associated proteins. ARNO has been shown to modulate the movement of mouse preadipocyte 3T3-L1 cells. Studies have reported that cytohesin-2 is overexpressed in pulmonary cancers and can also serve as biomarker of prognosis in hepatocellular carcinomas.
Monoclonal Anti-ARNO (Cytohesin-2) reacts specifically with ARNO, and does not cross-react with other members of the cytohesin family. The antibody associates with human and rat ARNO.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Mouse monoclonal anti-ARNO (Cytohesin-2) antibody can be used for western blot (5-10μg/mL using a whole extract of rat brain), microarray, indirect ELISA and immunocytochemistry applications.

To determine if ARNO binds to phosphoinositides, a protein-overlay assay was performed using monoclonal mouse anti-ARNO as the primary antibody for ARNO detection on membranes spotted with phosphoinositides and incubated with ARNO.

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