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A4825 Sigma

Monoclonal Anti-Assembly Protein 180 antibody produced in mouse

clone AP180-I, ascites fluid

Synonym: Anti-AP-3

Properties

Related Categories AS-ATG, Alphabetical Index, Antibodies, Primary Antibodies
species reactivity   human, bovine, rat
application(s)   immunocytochemistry: suitable
  immunoprecipitation: suitable
  western blot: 1:10,000 using rat brain extract
clone   AP180-I, monoclonal
antibody form   ascites fluid
isotype   IgG2b
mol wt   antigen mol wt 170-180 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... SNAP91(9892)
mouse ... Snap91(20616)
rat ... Snap91(65178)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

membrane protein fraction from bovine brain-coated vesicles.

General description

AP180 binds to clathrin triskelia with a stoichiometry of one per clathrin triskelion (trimer of heavy chains) and thereby induces assembly of clathrin into polyhedral cages. Monoclonal antibodies which react specifically with coated vesicle proteins are useful for studies on the intracellular distribution and structural relationship of clathrin-binding proteins. Mouse monoclonal anti-assembly protein 180 antibody binds to human AP180.

The antibody reacts in immunoblotting of bovine and rat brain preparations with the AP180 polypeptide (180 kDa) and with the proteolytic derivatives of AP180 (approximately 170 kDa). The antibody reacts with neuronal tissues and cultured cell lines. The antibody may be used in immunoaffinity purification. For immunocytochemistry applications, fixation with acetone or methanol is recommended.

Physical form

The product is provided as ascites fluid with 0.1% sodium azide as a preservative.

Application

Mouse monoclonal anti-assembly protein 180 antibody can be used for immunoblot and immunofluorescence studies. The antibody can also be used for immunocytochemistry and immunoprecipitation studies.

Price and Availability

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