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A4888 Sigma

Amphotericin B from Streptomyces sp.

~80% (HPLC), powder

Synonym: Fungizone

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Properties

Related Categories Antibiotics, Antibiotics A to Z, Antibiotics A-F, Antifungal, Biochemicals and Reagents,
Quality Level   PREMIUM
biological source   from Streptomyces sp.
InChI Key   APKFDSVGJQXUKY-INPOYWNPSA-N
assay   ~80% (HPLC)
form   powder
color   yellow to orange
solubility   DMF: 2-4 mg/mL
  DMSO: 30-40 mg/mL
  H2O: insoluble
Mode of action   cell membrane | interferes
antibiotic activity spectrum   fungi
  yeast
storage temp.   2-8°C

Description

Biochem/physiol Actions

Amphotericin B binds to sterols, forming pores in the membrane, and causing small molecules to leak out. The product is most effective against fungi and yeast.

Caution

Amphotericin B remains active for 3 days in culture at 37°C. At -20°C, and with protection from air and light, the product remains active for 5 years.

Packaging

1, 5, 100 g in glass bottle

100, 250, 500 mg in glass bottle

Preparation Note

Amphotericin B is insoluble in water at pH 6 to 7, but soluble in water at pH 2 or 11. It is soluble in DMSO (30-40 mg/mL) and in dimethylformamide (2-4 mg/mL). Aqueous solutions cannot be sterile filtered due to poor solubility.

Application

Amphotericin B is used to study the affects of the influx of calcium on antifungal activity, and to study the binding and kinetics of Amphotericin B to sterols in monolayers, aqueous solutions, and phospholipid bilayers . It is also commonly used in tissue culture as an antifungal supplement.
Amphotericin B from Streptomyces sp. has been used-
• for amphotericin perforated patch technique
• as a reference antifungal drug during MIC (minimum inhibitory concentration) agar dilution assay
• as a component of Arcobacter selective isolation broth

Other Notes

Keep container tightly closed in a dry and well-ventilated place. Product is sensitive to light and moisture. Keep in a dry place.

Protocols & Applications

Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

Price and Availability


Laboratory Gloves
Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
BU2625000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Antifungals

Antifungal compounds have been overshadowed by antibacterials in research interest and application due to the greater impact bacterial infections have had on health. Resistance to antibacterial drugs...
Chloe McClanahan
BioFiles 2009, 4.3, 10.
Keywords: Antibiotics, Antifungals, Cellular processes, Clinical, Fungicides, Gene expression, Research Reports, Transcription

Peer-Reviewed Papers
15

References

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