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A4929 Sigma

N,N′-Bis(acryloyl)cystamine

BioReagent, suitable for electrophoresis

Synonym: 2,2′-(Bisacrylamino)diethyl disulfide, BAC, Bis(2-acrylamidoethyl) disulfide

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Properties

Related Categories Acrylamides, Gel Cross-Linkers, Molecular Biology, Protein Electrophoresis, Proteomics,
InChI Key   DJVKJGIZQFBFGS-UHFFFAOYSA-N
product line   BioReagent
suitability   suitable for electrophoresis
storage temp.   −20°C

Description

Application

BAC is used as a reversible cross-linker for polyacrylamide gels, to simplify isolation of macromolecules separated on the gels. The disulfide linkage can be broken with a suitable reducing agent. A 1-g slice of a 4% (12:1) gel will dissolve in 5 mL of 100 mM DTT in TBE (pH 8.3) in less than 30 min.

BAC is used as a reversible cross-linker for polyacrylamide gels. The disulfide linkage can be broken with a suitable reducing agent. A 12:1 ratio of acrylamide:BAC is recommended for DNA purification. A one gram slice of a 4% gel will dissolve in 5 mL of 100 mM DTT, TBE solution (pH 8.3) in less than 30 min. The pH of 8.3 is optimum for rapid dissolution of the gel.

Packaging

5 g in glass bottle

Price and Availability


Laboratory Gloves
Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles
Peer-Reviewed Papers
15

References

Related Products

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Description

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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