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A4955 Sigma

Alkaline Phosphatase Stabilizing Buffer

liquid

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Description

Components

Stabilizing compounds in Tris buffered saline, pH 8.0, containing 1% bovine serum albumin and 15 mM sodium azide.

Packaging

1 L in poly bottle

100 mL in poly bottle

Preparation Note

The degree of stability realized is highly dependent upon the conjugate tested. Trials using each particular conjugate should be performed to determine the degree of stability that can be expected. It is recommended that this buffer constitute ≥95% of the final solution.

Application

Alkaline Phosphatase Stabilizing Buffer has been tested in ELISA systems. The product has been specially made for the stable dilution of alkaline phosphatase-antibody conjugates.It can be used for a series of experiments to achieve consistent results.

General description

Alkaline Phosphatase Stabilizing Buffer can extend the shelf life of alkaline phosphate conjugates.

Price and Availability


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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Antibody Explorer - Search 80,000 Antibodies at Sigma-Aldrich

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

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