|Related Categories||Apoptosis Detection, Apoptosis and Cell Cycle, Cell Biology, Cell Signaling and Neuroscience, Reagents More...|
|form||buffered aqueous solution|
|extent of labeling||1-3 mol Cy3.18 per mol annexin V|
|shipped in||wet ice|
|Gene Information||human ... ANXA5(308)|
For detection of apoptotic cells by fluorescence microscopy.
Solution in 50 mM Tris-HCl, pH 7.5, containing 100 mM NaCl.
Annexins are ubiquitous homologous proteins that bind phospholipids in the presence of calcium. The cellular changes involved in the apoptotic process include loss of phospholipid asymmetry during the early stages. This phenomenon is universal and is not limited to stimulus or to mammalian cells, but also occurs in insect and plant cells. In living cells, phosphatidylserine is transported to the inside of the lipid bilayer by the Mg2+ATP dependent enzyme, aminophospholipid translocase. At the onset of apoptosis, phosphatidylserine becomes translocated to the external surface of the cell membrane. Since the movement of phosphatidylserine from the internal membrane surface to the external surface is an early indicator of apoptosis, annexin V and its conjugates that interact strongly and specifically with phosphatidylserine may be used to detect apoptosis. Annexin V conjugates can be used to detect apoptotic cells significantly earlier than DNA-based assays. Fluorescent dye labeled annexin V have applications in flow cytometry, fluorescence microscopy, and laser scanning cytometry.
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Early redistribution of plasma membrane phosphatidylserine is a general feature of apoptosis regardless of the initiating stimulus: inhibition by overexpression of Bcl-2 and Abl. Martin, S.J., et al. J. Exp. Med. 182, 1545-1556, (1995)
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