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A50120 Sigma

DEAE–Sephadex®

Synonym: Diethylaminoethyl Sephadex®

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Properties

Related Categories Anion Exchange Media, Ion Exchange Chromatography, Molecular Biology, Protein Chromatography, Proteomics More...
bead size   40-125 μm (dry)
pore size   ~200,000 Da exclusion limit
capacity   3-4 meq/g binding capacity

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Packaging

50, 100 g in poly bottle

Application

DEAE Sephadex can be used in ion exchange chromatography for purifying and isolating proteins. In has been used to identify and label an insulin activated nitric oxide synthase inhibitor (protein) in acute myocardial infarction (AMI) patients.

Legal Information

Sephadex is a registered trademark of GE Healthcare

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
What is the procedure for swelling Product A50120, DEAE–Sephadex® resin?
Weigh out the required amount of dry powder and suspend it in the buffer of choice. Although the swelling volume will be specific to the binding buffer used, generally 1 gram of powder will swell to 25 mL final volume of medium. Excess binding buffer should be used. Swell the resin for 1-2 days at room temperature or 2 hours at 100°C over boiling water. After swelling, wash the resin on a Buchner funnel with binding buffer. Prepare a slurry with 75% swelled medium/25% binding buffer.
What procedures are used for elution when using Product A50120, DEAE–Sephadex® resin?
For DEAE Sephadex, elution is achieved using either an increasing salt gradient or a decreasing pH gradient.
How can Product A50120, DEAE–Sephadex® resin be regenerated?
Regeneration is generally accomplished by washing with high ionic strength buffer or decreasing/increasing pH. The resin is then re-equilibrated in binding buffer.
How can Product A50120, DEAE–Sephadex® resin, be stored?
Store swollen medium refrigerated in the presence of a preservative such as 20% Ethanol. Sodium azide or thimerosal should not be used.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles
Peer-Reviewed Papers
15

References

Related Products

replaced by

Product #

Description

Add to Cart

GE17-0180-02 DEAE-Sephadex® A-50, GE Healthcare, 17-0180-02, pack of 500 g
GE17-0180-01 DEAE-Sephadex® A-50, GE Healthcare, 17-0180-01, pack of 100 g

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