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A5043 Sigma

Adenosine Deaminase from bovine spleen

Type X, buffered aqueous glycerol solution, ≥130 units/mg protein

Synonym: Adenosine aminohydrolase

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Properties

Related Categories 3.5.x.x Acting on C-N other than peptides, 3.x.x.x Hydrolases, Biochemicals and Reagents, Cell Biology, Enzyme Class Index,
type   Type X
form   buffered aqueous glycerol solution
cation traces   NH4+: ≤10 μg/mg protein
foreign activity   alkaline phosphatase ≤0.01%
  guanase ≤0.01%
  nucleoside phosphorylase ≤0.1%
storage temp.   2-8°C
Gene Information   cow ... ADA(280712)

Description

Analysis Note

Protein determined by biuret.

Unit Definition

One unit will deaminate 1.0 μmole of adenosine to inosine per min at pH 7.5 at 25 °C.

Physical form

Solution in 50% glycerol, 5 mM potassium phosphate, pH 6.0

Application

Adenosine deaminase is useful in various molecular biology assays, such as glycerol release assays . Adenosine deaminase is a potential target for treatments of combined immunodeficiency disease.

Biochem/physiol Actions

Adenosine deaminase is a purine catabolic enzyme which catalyzes the deamination of adenosine and 2′-deoxyadenosine with approximately equal specificity. It is ubiquitous in mammalian tissue, and deficiency in adenosine deaminase has been associated with severe combined immunodeficiency disease.

General description

Molecular weight: 32.5-33 kDa

pI: 4.85

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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