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A5044 Sigma

Monoclonal Anti-α-Actinin antibody produced in mouse

clone BM-75.2, ascites fluid

Properties

Related Categories AC-AC, Alphabetical Index, Antibodies, Antibodies for Cardiac Stem Cells, Antibodies for Cell Biology,
species reactivity   mouse, bovine, human, chicken
application(s)   indirect immunofluorescence: 1:200 using cultured chicken fibroblasts
  microarray: suitable
  western blot: suitable
clone   BM-75.2, monoclonal
antibody form   ascites fluid
isotype   IgM
mol wt   antigen mol wt 100 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... ACTN1(87)
mouse ... Actn1(109711)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

cytoskeletal fraction of bovine mammary gland epithelial (BMGE) cells.

General description

α-actinin is a 100kD actin binding protein found in muscle as well as non-muscle cells. In smooth muscle, α-actinin is identified in dense bodies and plaques characteristic of tissues whereas it is associated with z-discs that define muscle sarcomas in normal skeletal muscle. Monoclonal anti-α-actinin antibody can be used in immunoblotting to study the membrane anchorage sites and immunochemical identification of α-actinin. It can also be used in immunofluorescence to study the localization of α-actinin in cultured cells and tissues. Mouse anti-α-actinin antibody reacts specifically with chicken fibroblasts. The product has also shown reactivity for mouse and human cells.

Application

Immunofluorescence of cardiomyocytes from neonatal and adult mouse hearts was performed using monoclonal mouse anti-α actinin (clone EA-53) at 1:1000.

May be used in the study of skeletal muscle organization and in studies of the membrane anchorage sites of actin.

Monoclonal anti-α-actinin antibody (diluted 1: 500) can be used in native protein binding assays. It can also be used in western blot, microarray, immunohistochemistry and in Immunofluorescence microscopy.

Price and Availability


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