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A5060 Sigma

Anti-Actin (20-33) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Properties

Related Categories AC-AC, Actin Antibodies, Alphabetical Index, Antibodies, Antibodies for Cell Biology,
species reactivity   wide range (animal), invertebrates
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using chicken or rat tissue sections
  indirect immunofluorescence: 1:200 using cultured human fibroblasts
  microarray: suitable
  western blot: 1:250 using using rat brain or chicken muscle extracts
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 42 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... ACTA1(58)
mouse ... Acta1(11459)
rat ... Acta1(29437)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

N-terminal actin peptide (amino acid residues 20-33) attached to a multiple antigen peptide (MAP) backbone.

General description

Actin is a highly conserved protein that is a major component of both the cytoskeletal and contractile structures in all cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. Actin can be found in two different forms of aggregation, the globular or the fibrillar state, and at least six distinct isoforms occur in vertebrates. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three α actins (skeletal, cardiac, smooth), one β actin (β-non-muscle) and two γ actins (γ smooth muscle and γ non-muscle).

The antibody shows a broad reactivity among actin isoforms and across a range of organisms. It specifically stains typical stress fibers in cultured cells using an indirect immunofluorescent labeling technique and can be used as a probe of the N-terminal region of actin.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative.

Application

Homogenates from limb tissue of E13.5 mouse embryos were analyzed by western blot using rabbit anti-actin as a primary antibody at a dilution of 1:8000.

Rabbit Anti-Actin (20-33) antibody has been used for immunoblotting assays. The product can also be used for immunohistochemistry, indirect immunofluorescence and microarray analyses.

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

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