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A5062 Sigma

Anti-Guinea Pig IgG (whole molecule)−Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous glycerol solution

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Properties

Related Categories Alkaline Phosphatase, Alphabetical Index, Antibodies, GR-GZ, Guinea Pig Secondary Antibodies and Conjugates,
biological source   goat
antibody form   affinity isolated antibody
clone   polyclonal
form   buffered aqueous glycerol solution
application(s)   direct ELISA: 1:30,000
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
  western blot: 1:30,000
conjugate   alkaline phosphatase conjugate
shipped in   wet ice
storage temp.   2-8°C

Description

Immunogen

Purified guinea pig IgG.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1 mM MgCl2, 10 mM glycine, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide

Application

Anti-guinea pig IgG (whole molecule) −alkaline phosphatase antibody can be used in direct ELISA (diluted 1:30,000) and dot blot.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Western blot analysis of SF9 cells was performed using alkaline phosphatase conjugated goat anti-guinea pig IgGat 1:10000 as the secondary.

General description

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . In guinea pig IgG is subdivided into-IgG1 and IgG2. IgG2 can bind to Fc receptor of macrophages and monocytes. Anti-guinea pig IgG (whole molecule) −alkaline phosphatase antibody can be used in immunohistology (diluted 1:50). Goat anti-guinea pig IgG (whole molecule) −alkaline phosphatase antibody reacts specifically with guinea pig IgG.

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Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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Peer-Reviewed Papers
15

References

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