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A5147 Sigma

L-Amino Acid Oxidase from Crotalus atrox (Western Diamondback Rattlesnake)

Type VI, dried venom, ≥0.10 unit/mg solid

Synonym: L-AAO, L-Amino acid: oxygen oxidoreductase (deaminating)

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Description

Unit Definition

One unit will oxidatively deaminate 1.0 μmole of L-phenylalanine per min at pH 6.5 at 37 °C. (L-Leucine is deaminated at the same rate at pH 7.8 at 37 °C.)

Application

L-amino acid oxidase is used to convert L-amino acids to their corresponding α-keto acids. Product A5147 is from Crotalus atrox. One unit will oxidatively deaminate 1.0 μmole of L-phenylalanine per min at pH 6.5 at 37 °C. L-amino acid oxidase, from Sigma, has been used in leucine aminopeptidase (LAP) activity assays .

Biochem/physiol Actions

L-amino acid oxidase is involved in various metabolic pathways such as alanine and aspartate metabolism, methionine metabolism, valine, leucine and isoleucine degradation, tyrosine metabolism, phenylalanine metabolism, tryptophan metabolism, phenylalanine, tyrosine and tryptophan biosynthesis, and alkaloid biosynthesis .

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Safety & Documentation

Safety Information

Symbol 
GHS06  GHS06
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 3462 6.1 / PGI
WGK Germany 
3
Protocols & Articles

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15

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