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A5159 Sigma

Aminopterin

Hybri-Max, 50 ×, γ-irradiated, lyophilized powder, BioXtra, suitable for hybridoma

Synonym: (S)-2-{4-[(2,4-Diaminopteridin-6-yl)methylamino]benzamido}pentanedioic acid, 4-Amino-PGA, 4-Aminofolic acid, 4-Aminopteroyl-L-glutamic acid

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Properties

Related Categories Cell Culture, Conditioned Media, Media Supplements and Reagents, Hybridoma Platform, Hybridoma Reagents, Reagents and Supplements,
grade   Hybri-Max
InChI Key   TVZGACDUOSZQKY-LBPRGKRZSA-N
sterility   γ-irradiated
product line   BioXtra
form   lyophilized powder
concentration   50 ×
impurities   endotoxin, tested
solubility   : soluble10 mL/vial, clear, red (in TC Medium)
absorption   ε1M/261 nm, 0.1 M NaOH 25,700
  ε1M/282 nm, 0.1 M NaOH 24,500
  ε1M/373 nm, 0.1 M NaOH 8,100
suitability   suitable for hybridoma
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... FPGS(2356)
mouse ... Fpgs(14287)

Description

Application

Aminopterin concentrate supplement for hybridoma cell culture applications.

Linkage

More potent, but more toxic, than methotrexate.

Reconstitution

Reconstitute contents of vial with 10 mL sterile cell culture medium. Stock solution is sufficient to prepare 500 mL medium. Final working concentration: 0.4 μM aminopterin.

Biochem/physiol Actions

Folic acid antagonist. Aminopterin is actively transported into cells by the folate transporter. In the cell, it is converted to a high molecular weight polyglutamate metabolite by folylpolyglutamate synthase that, in turn, binds to dihydrofolate reductase and inhibits its activity. Aminopterin-polyglutamate is degraded intracellularly by γ-glutamyl hydrolase.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Other Notes

Tandem Mass Spectrometry data independently generated by Scripps Center for Metabolomics is available to view or download in PDF. A5159.pdf Tested metabolites are featured on Scripps Center for Metabolomics METLIN Metabolite Database. To learn more, visit sigma.com/metlin.

Price and Availability


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Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 2811 6.1 / PGI
WGK Germany 
3
RTECS 
MA1050000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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A3411 Aminopterin, powder, BioReagent, suitable for cell culture
92210 Timestrip Plus 0 °C

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