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A5170 Sigma

Anti-Avidin antibody produced in rabbit

whole antiserum

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Properties

Related Categories ATH-AZ, Alphabetical Index, Antibodies, Miscellaneous, Primary Antibodies More...
biological source   rabbit
antibody form   whole antiserum
clone   polyclonal
contains   15 mM sodium azide
application(s)   immunoelectrophoresis: suitable
  indirect ELISA: 1:100,000
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Analysis Note

This antisera is evaluated for performance and specificity by immunodiffusion and immunoelectrophoresis.

Immunogen

avidin

Preparation Note

treated to remove lipoproteins

Physical form

Supplied as a liquid containing 15mM sodium azide as preservative

Application

Rabbit anti-avidin antibody can be used for indirect ELISA (1:100,000) and immunoelectrophoresis.

The presence of Scavidin in BT4C glioma cells was assessed by western blot using a rabbit anti-avidin antibody as the primary at a dilution of 1:4000. Lehtolainen, P. (2002) Cloning and Characterization of Scavidin, a Fusion Protein for the Targeted Delivery of Biotinylated Molecules. J. Biol. Chem. 277:8545-8550.

General description

Avidin is homotetrameric protein (68kDa) that is obtained from egg whites. This egg protein binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.
Rabbit anti-avidin antibody is immunospecific for egg white avidin by immunoelectrophoresis versus egg white avidin. No reactivity is observed versus streptavidin.

Price and Availability


Detect Your Unseen Protein
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

References

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