A5228 Sigma

Monoclonal Anti-Actin, α-Smooth Muscle antibody produced in mouse

clone 1A4, purified immunoglobulin

DOWNLOAD MSDS (PDF)

Synonym: SMA

Properties

Related Categories 1-1B, AC-AC, Actin Antibodies, Alphabetical Index, Antibodies,
species reactivity   rabbit, mouse, guinea pig, chicken, goat, sheep, snake, human, frog, rat, canine, bovine
application(s)   immunocytochemistry: suitable using smooth muscle cells
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using smooth muscle cells
  indirect ELISA: suitable
  indirect immunofluorescence: 5-10 μg/mL using blood vessels in formalin-fixed, paraffin-embedded human tonsil or appendix tissue sections
  microarray: suitable
  western blot: suitable using smooth muscle cells
clone   1A4, monoclonal
antibody form   purified immunoglobulin
isotype   IgG2a
mol wt   antigen mol wt ~42 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... ACTA2(59)
mouse ... Acta2(11475)
rat ... Acta2(81633)

Description

Immunogen

N-terminal synthetic decapeptide of α-smooth muscle actin.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

The antibody (also known as anti-α-Sm-1) is specific for the single isoform of α-smooth muscle actin. It reacts specifically with α-smooth muscle actin in immunoblotting assays and labels smooth muscle cells in frozen or formalin-fixed, paraffin-embedded tissue sections.

Application

Immunocytochemistry was performed on smooth muscle cells from bovine aortas using the monoclonal anti-ACTA2 antibody. Cells were first grown on glass cover slips and fixed in 50% acetone/EtOH for 10 minutes at 4 degrees.1

Paraffin embedded sections of rat testis tissue grafts were immunohistochemically stained with mouse monoclonal anti-smooth muscle actin.
IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Price and Availability

Anti-Mouse 2° Antibody Best Sellers


Prod. No.ConjugateSecondary Antibody Description
A4416HRPAnti-Mouse IgG (whole molecule)-Peroxidase
A3562APAnti-Mouse IgG (whole molecule)-Alkaline Phosphatase
F0257FITCAnti-Mouse IgG (whole molecule)-FITC
B7264BiotinAnti-Mouse IgG (whole molecule)-Biotin





Customers Also Viewed

Anti-Smooth Muscle α-Actin antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Monoclonal Anti-Actin, α-Smooth Muscle - Alkaline Phosphatase antibody produced in mouse

clone 1A4, purified immunoglobulin, buffered aqueous glycerol solution

Monoclonal Anti-Actin, α-Smooth Muscle - Cy3<SUP>™</SUP> antibody produced in mouse

clone 1A4, purified immunoglobulin, buffered aqueous solution

clone 1A4, purified immunoglobulin, buffered aqueous solution

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?