Synonym: SMA
Popular Documents: Datasheet (PDF)
| Related Categories | 1-1B, AC-AC, Actin Antibodies, Alphabetical Index, Antibodies, |
| species reactivity | rabbit, mouse, guinea pig, chicken, goat, sheep, snake, human, frog, rat, canine, bovine |
| application(s) | immunocytochemistry: suitable using smooth muscle cells |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using smooth muscle cells | |
| indirect ELISA: suitable | |
| indirect immunofluorescence: 5-10 μg/mL using blood vessels in formalin-fixed, paraffin-embedded human tonsil or appendix tissue sections | |
| microarray: suitable | |
| western blot: suitable using smooth muscle cells | |
| clone | 1A4, monoclonal |
| antibody form | purified immunoglobulin |
| isotype | IgG2a |
| mol wt | antigen mol wt ~42 kDa |
| shipped in | dry ice |
| storage temp. | −20°C |
| Gene Information |
human ... ACTA2(59) mouse ... Acta2(11475) rat ... Acta2(81633) |
N-terminal synthetic decapeptide of α-smooth muscle actin.
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
The antibody (also known as anti-α-Sm-1) is specific for the single isoform of α-smooth muscle actin. It reacts specifically with α-smooth muscle actin in immunoblotting assays and labels smooth muscle cells in frozen or formalin-fixed, paraffin-embedded tissue sections.
Immunocytochemistry was performed on smooth muscle cells from bovine aortas using the monoclonal anti-ACTA2 antibody. Cells were first grown on glass cover slips and fixed in 50% acetone/EtOH for 10 minutes at 4 degrees.1
Paraffin embedded sections of rat testis tissue grafts were immunohistochemically stained with mouse monoclonal anti-smooth muscle actin.
IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.
To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/acti
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