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A5230 Sigma

Anti-ARF6 antibody, Mouse monoclonal

clone ARFAG, purified from hybridoma cell culture

Synonym: Anti-ADP-Ribosylation Factor 6

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Properties

Related Categories AR-AR, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Neurobiology,
species reactivity   canine, rat, bovine, human
application(s)   western blot: 1-2 μg/mL using MDCK total cell extract.
clone   ARFAG, monoclonal
concentration   2 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 18 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... ARF6(382)
rat ... Arf6(79121)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 141-155 of human ARF6.

General description

ARF6 encodes a member of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and modulate vesicular transport.

ARF6 is a member of the ADP-ribosylation factor (ARF) family. It is specifically involved in endocytosis of plasma membrane proteins.

Mouse monoclonal anti-ARF6 antibody binds to human, bovine, canine and rat ARF6.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Mouse Monoclonal Anti-ARF6 antibody can be used for western blot assays at 1-2μg/ml using MDCK cell extracts. The antibody can also be used for ELISA and immunocytochemistry.

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  3
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Peer-Reviewed Papers
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