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A5278 Sigma

Anti-Mouse IgG (whole molecule)–Peroxidase antibody produced in goat

IgG fraction of antiserum, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, MO-MR, Mouse Secondary Antibodies and Conjugates, Peroxidase Labeled Antibodies,
biological source   goat
antibody form   IgG fraction of antiserum
clone   polyclonal
form   buffered aqueous solution
application(s)   direct ELISA: 1:10,000
conjugate   peroxidase conjugate
shipped in   dry ice
storage temp.   −20°C

Description

Immunogen

Purified mouse IgG.

Packaging

1 mL in glass bottle

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin and 0.01% thimerosal.

Application

Anti-mouse IgG (whole molecule)-peroxidase antibody (diluted 1: 300) can be used as a secondary antibody in immunohistochemistry. It can also be used in Ouchterlony double diffusion and immunoelectrophoresis.

Western blot analysis using HRP conjugated goat anti-mouse IgG as the secondary antibody at a dilution of 1:3000 in BSA/PBS was performed to detect IgG antibodies against outer-membrane vexicle proteins.

General description

IgG antibodies are the smallest antibody isotype found in blood and extracellular fluid. These antibodies are capable of fighting bacterial and viral infection. They also regulate several functions like placental transport, cell surface-receptor binding and complement activation. Anti-mouse IgG (whole molecule)-peroxidase antibody can be used in ELISA (diluted 1: 200 in PBST) . It can also be used in western blotting. Goat anti-mouse IgG-peroxidase antibody reacts specifically with mouse IgA, IgG1, IgG2a, IgG2b, IgG3 and IgM myeloma proteins.

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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Antibody Explorer - Over 80,000 Products

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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06693 Timestrip Plus -20 °C

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