EMAIL THIS PAGE TO A FRIEND
A5304 Sigma

Agarose

for electrophoresis

Purchase

Properties

Related Categories Agarose, Core Bioreagents, Life Science Reagents for DNA/RNA Electrophoresis, Life Science Reagents for Northern and Southern Blotting, Life Science Reagents for Protein Electrophoresis,
grade   for electrophoresis
impurities   ≤7% water
ash   ≤1.5%
turbidity   ≤4 NTU (1.5% gel)
EEO   ≤0.40
transition temp   gel point 36 °C ±1.5 °C
gel strength   ≥700 g/cm2 (1% gel)
anion traces   sulfate (SO42-): ≤0.2%

Description

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Packaging

100 g in glass bottle

Suitability

Suitable for counterimmunoelectrophoresis and immunoelectrophoretic techniques with significant cathodal migration.

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.




Hybridization
Safety & Documentation

Safety Information

WGK Germany 
3
Protocols & Articles

Related Content

Agarose Selection Guide

Agarose Selection Guide – Click on the Product Number to Purchase Specialty Agarose – Click on the Product Number to Purchase
Keywords: Cell culture, Electrophoresis, Immunodiffusion, Immunoelectrophoresis, Molecular biology, Protein electrophoresis, Separation

Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?