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A5304 Sigma

Agarose

for electrophoresis

Properties

Related Categories Agarose, Core Bioreagents, Life Science Reagents for DNA/RNA Electrophoresis, Life Science Reagents for Northern and Southern Blotting, Life Science Reagents for Protein Electrophoresis,
grade   for electrophoresis
impurities   ≤7% water
ash   ≤1.5%
turbidity   ≤4 NTU (1.5% gel)
EEO   ≤0.40
transition temp   gel point 36 °C ±1.5 °C
gel strength   ≥700 g/cm2 (1% gel)
anion traces   sulfate (SO42-): ≤0.30%

Description

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Suitability

Suitable for counterimmunoelectrophoresis and immunoelectrophoretic techniques with significant cathodal migration.

Price and Availability


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