• USA Home
  • A5405 - Avidin-ferritin labeled

EMAIL THIS PAGE TO A FRIEND
A5405 Sigma

Avidin-ferritin labeled

Avidin from egg white, lyophilized powder

Purchase

Properties

Related Categories Antibodies, Avidin Reagents, Biotin/Avidin, Supplementary Products
biological source   Avidin from egg white
form   lyophilized powder
composition   Protein, ~30%
conjugate   ferritin conjugate
storage temp.   −20°C

Description

Analysis Note

Actual ferritin: avidin ratio given on label.

Packaging

Package size based on protein content

Preparation Note

Labeled with equine spleen ferritin.
Prepared by modification of the procedure of Sullivan, M.J., et al., FEBS Lett., 95, 311 (1978).

Physical form

Lyophilized powder containing Tris buffer salt

Application

The product has been used to detect biotin bound to avidin tags in Escherichia coli strains.

General description

Avidin is homotetrameric protein (68kDa) that is obtained from egg whites. This egg protein binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.

Price and Availability


Detect Your Unseen Protein
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Related Content

Antibody Explorer - Over 80,000 Products

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?