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A5441 Sigma

Monoclonal Anti-β-Actin antibody produced in mouse

clone AC-15, ascites fluid

Synonym: Monoclonal Anti-β-Actin

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Properties

Related Categories AC-AC, Actin Antibodies, Alphabetical Index, Antibodies, Antibodies for Cell Biology,
species reactivity   canine, guinea pig, Hirudo medicinalis, feline, pig, mouse, carp, chicken, sheep, rabbit, rat, human, bovine
does not react with   Dictyostelium discoideum
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
  indirect ELISA: suitable
  indirect immunofluorescence: 1:1,000-1:2,000 using cultured human or chicken fibroblasts
  western blot: 1:5,000-1:10,000 using cultured human or chicken fibroblast cell extracts
clone   AC-15, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt 42 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... ACTB(60)
mouse ... Actb(11461)
rat ... Actb(81822)
biological source   mouse
conjugate   unconjugated
Featured Industry   Research Pathology

Description

Immunogen

slightly modified β-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.

General description

In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used.

The antibody can be used for staining of acetone-fixed frozen sections, EM preparations, and microinjection experiments. B5, ethanol, methacam, or Bouin′s solutions can be used as fixatives. The epitope recognized by the antibody is resistant to formalin-fixed and paraffin-embedding.

The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in cellular process including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms characterized by electrophoresis and amino acid sequence analysis. Four of them represent the differentiation markers of muscle tissues and two are found practically in all cells. There are three α-actins (skeletal, cardiac, and smooth muscle), one β-actin (β-nonmuscle), and two γ-actins (γ-smooth muscle and γ-nonmuscle). Actin isoforms show >90% overall sequence homology, but only 50–60% homology in their NH2-terminal residues.

Physical form

Supplied as ascites fluid with 15mM sodium azide as a preservative.

Application

Monoclonal mouse anti-actin antibody was used as a loading control for western blot analysis of immunoprecipitated proteins from rat dorsal root ganglion cocultures.

Monoclonal mouse anti-actin was used as a loading control for western blot analysis of rat liver protein lysates.

Mouse Monoclonal Anti-β-Actin antibody has been used as a control for protein arrays and immunoblot assays. The antibody can also be used for IHC, indirect ELISA and indirect immunofluorescence (1:1,000-1:2,000).

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

Articles

Antibodies to Actin Gene Family Isoforms

The Actin Gene Family Actin and myosin are the two major cytoskeletal proteins implicated in cell motility. As constituents of many cell types, actin and myosin are involved in a myriad of cellular p...
Keywords: Western blot

Antibodies to Actin and GAPDH

Actin and myosin are the two major cytoskeletal proteins implicated in cell motility. As constituents of many cell types, actin and myosin are involved in a myriad of cellular process including locom...
Leigh Gaskill
BioFiles 2008, 3.6, 10.
Keywords: Biofiles, Immunofluorescence, PAGE, Western blot

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Peer-Reviewed Papers
15

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06693 Timestrip Plus -20 °C

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