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  • A5545 - Anti-Guinea Pig IgG (whole molecule)−Peroxidase antibody produced in rabbit

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A5545 Sigma

Anti-Guinea Pig IgG (whole molecule)−Peroxidase antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, GR-GZ, Guinea Pig Secondary Antibodies and Conjugates, Peroxidase Labeled Antibodies,
biological source   rabbit
antibody form   affinity isolated antibody
clone   polyclonal
form   buffered aqueous solution
species reactivity   guinea pig
application(s)   direct ELISA: 1:50,000-1:80,000
  dot blot: 1:50,000-1:100,000
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400-1:800
conjugate   peroxidase conjugate
shipped in   dry ice
storage temp.   −20°C

Description

Immunogen

guinea pig IgG

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.01% thimerosal

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)
Western Blotting (1 paper)

BHK-21 cell lysates were analyzed by western blot using HRP-conjugated rabbit anti-guinea pig IgG as the secondary antibody at a 1:10000 dilution in 0.1% tween/PBS.

Rabbit anti-guinea pig IgG (whole molecule)-Peroxidase antibody has been used for western blot and immunohistochemistry assays. The product can also be used for dot blot (1:50,000-1:100,000) and direct ELISA (1:50,000-1:80,000).

General description

IgGs are glycoprotein antibodies that modulate several immune responses. Guinea pig IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-guinea pig IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Rabbit anti-guinea pig IgG (whole molecule)-peroxidase antibody binds to guinea pig IgG.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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Peer-Reviewed Papers
15

References

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