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A5560 Sigma

Anti-Water Channel Aquaporin 1 antibody produced in rabbit

IgG fraction of antiserum, lyophilized powder

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Aquaporin (Water) Channels, Antibodies to Ion Channels,
species reactivity   rat, human
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
  western blot (chemiluminescent): 1:400-1:800
clone   polyclonal
antibody form   IgG fraction of antiserum
form   lyophilized powder
storage temp.   −20°C
Gene Information   human ... AQP1(358)
rat ... Aqp1(25240)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 243-261 of human aquaporin 1 conjugated to KLH with glutaraldehyde. The epitope is identical in human, rodent, and cattle and only slightly different in frog.

General description

Aquaporins are transmembrane proteins composed of 6 α helices that regulate the flow of water across the membrane. AQP1 is highly expressed on the apical membrane of kidneys.

Rabbit Anti-Water Channel Aquaporin 1 antibody binds to human and rat aquaporin 1.

Physical form

Lyophilized from phosphate buffered saline, pH 7.4, and 0.025% sodium azide

Preparation Note

Isolated on immobilized Protein A

Application

Rabbit Anti-Water Channel Aquaporin 1 antibody has been used for immunocytochemical assays. The product can also be used for immunohistochemistry and western blot.

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
RIDADR  NONH for all modes of transport
WGK Germany  3
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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

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R4505 Rabbit Serum, USDA approved, sterile-filtered, suitable for cell culture

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