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A5598 Sigma

Anti-c-Myc−Peroxidase antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-c-Myc antibody produced in rabbit

Properties

Related Categories c-myc, Alphabetical Index, Antibodies, Antibodies and Conjugates, Antibodies for Cell Biology,
species reactivity   human
application(s)   western blot: 1:5,000
clone   polyclonal
concentration   1.8-4 mg/mL
antibody form   affinity isolated antibody
form   buffered aqueous solution
storage temp.   −20°C
Gene Information   human ... MYC(4609)
biological source   rabbit
conjugate   peroxidase conjugate

Description

Immunogen

peptide corresponding amino acids 408-425 of the human c-myc proto-oncogene, conjugated to maleimide-activated KLH through a C-terminal added cysteine residue.

General description

The Anti-c-Myc peroxidase conjugate rabbit antibody reacts with c-Myc tagged fusion proteins. It recognizes the c-Myc tag sequence expressed at either the amino- or the carboxyl-terminus.

Physical form

Solution in 0.01 M sodium phosphate buffer saline, pH 7.4, containing 0.01% thimerosal.

Specificity

N-terminal or C-terminal c-Myc (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) tagged fusion proteins.

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Application

Antibody is suitable for immunoblotting.

Features and Benefits

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