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A5719 Sigma

Anti-FITC-Alkaline Phosphatase antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution



Related Categories Alphabetical Index, Antibodies, Colorimetric, FK-FO, Molecular Biology,
biological source   rabbit
antibody form   IgG fraction of antiserum
clone   polyclonal
form   buffered aqueous solution
application(s)   dot blot: 1:2,000 using fluoresceinated nucleic acid probes
isotype   IgG
conjugate   alkaline phosphatase conjugate
shipped in   wet ice
storage temp.   2-8°C



Do not freeze.


FITC conjugated to BSA


5 mL in poly bottle

Physical form

Solution in Tris-buffered saline, pH 8.0, containing 1% bovine serum albumin and 0.1% sodium azide.


Immunohistochemistry of paraffin embedded, 2% paraformaldhyde fixed adult rectal sections was performed using alkaline phosphatase-conjugated rabbit anti-FITC antibody (A4843) at a 1:100 dilution.

Rabbit Anti-FITC-Alkaline Phosphatase antibody has been used to detect CFSE labelled cells using immunohistochemical techniques. The antibody can also be used for dot blot (1:2,000 using fluorinated nucleic acid probes).

The conjugate is formulated to detect FITC-labeled, nucleic acid probes in membrane-based blotting applications.

General description

FITC (fluorescein isothiocyanate) is a fluorophore that absorbs ultraviolet or blue light and emits visible yellow-green light. Fluorescent labeling allows quick and precise localization of antigen-antibody associations in tissues and cells. FITC is a widely used antibody marker in immunofluorescence assays, as protein-FITC conjugation is comparatively a simple task and usually does not interfere with protein function. FITC is also utilized as a hapten to label proteins. Thus, anti-FITC antibodies can facilitate the analyses of FITC labeled proteins and antibody responses to haptens.

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Safety Information

WGK Germany 
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

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