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A5838 Sigma

Anti-Glutathione-S-Transferase (GST)–Alkaline Phosphatase Conjugate antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-Glutathione-S-Transferase, Anti-GST

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Properties

Related Categories Alkaline Phosphatase Conjugates, Alphabetical Index, Antibodies, Antibodies and Conjugates, Antibodies to Peptide and Protein Fusion Tags,
biological source   rabbit
antibody form   IgG fraction of antiserum
clone   polyclonal
form   buffered aqueous solution
mol wt   antigen mol wt 27.5  kDa
application(s)   direct ELISA: 1:400 using using wells coated with 5 μg/mL GST in carbonate buffer
  western blot (chemiluminescent): 1:20,000
conjugate   alkaline phosphatase conjugate
shipped in   wet ice
storage temp.   2-8°C

Description

Immunogen

recombinant GST from Schistosoma japonicum expressed in E. coli.

Physical form

Solution supplied in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% BSA, and 15 mM sodium azide.

Application

Alkaline phosphatase (AP) conjugated anti-GST antibodies have been used as probes for western blot assays. The product can also be used for direct ELISA (1:400).

Purification of GST-SfMannIII from Sf9 insect cells was confirmed by western blot using rabbit anti-GST antibody. Gst-SfMan I was purified from over-expressing SF/SWT-1 cells and ran on SDS-page fell for western blot analysis using rabbit anti-GST as the primary antibody. Alkaline phosphatase-conjugated goat anti-rabbit IgG (Sigma) was used as the secondary antibody.

General description

Recombinant target proteins are often expressed as a fusion product with Glutathione-S-Transferase (GST) tags using various expression vector constructs. Thus, antibodies directed against the GST tags of the recombinant constructs can facilitate the purification and study of target proteins.

The antibody is specific for native as well as denatured-reduced forms of glutathione-S-transferase from Schistosoma japonicum. Anti-GST may be used in various immunoassays to identify the expression of GST fusion proteins.

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Antibody Basics

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