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A5844 Sigma

Monoclonal Anti-c-Abl antibody produced in mouse

clone ABL-148, ascites fluid

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Cycle, Antibodies for Kinase/Phosphatase Biology,
species reactivity   monkey, mouse, rat, human, bovine
application(s)   flow cytometry: suitable
  immunocytochemistry: suitable
  immunoprecipitation: suitable
  microarray: suitable
  western blot: 1:2,000 using human melanoma cell extract
clone   ABL-148, monoclonal
antibody form   ascites fluid
isotype   IgG2a
mol wt   antigen mol wt 145 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... ABL1(25)
mouse ... Abl1(11350)
rat ... Abl1(311860)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

recombinant c-Abl, SH2 domain.

General description

c-Abl is a proto-oncogene product that belongs to Tyr protein kinase family. It has a crucial role in different cellular processes like- differentiation, adhesion and regulates DNA damage-induced apoptosis. Monoclonal anti-c-Abl antibody can be used for studying the mechanism of signaling pathways involving c-Abl. It may also be used for immunoprecipitation and immunocytochemistry. Mouse anti-c-Abl antibody reacts specifically with an epitope present in the SH2 domain of the c-Abl. The product has also shown reactivity for c-Abl of monkey, rat, bovine, mouse and human.

Antigen Background

c-Abl is a non-receptor tyrosine kinase with both cytoplasmic and nuclear functions. The Abl oncogen has been implicated in several human leukemias including nearly all chronic myelocytic leukemias.

Application

Hippocampal extracts from rats were analyzed by western blot using anti-phospho TYR412 c-Abl as the primary antibody at a dilution of 1:1000.

Monoclonal anti-c-Abl antibody can be used in western blotting (diluted 1:2000) using melanoma cell extract from human. It can also be used in microarray and flow cytometry.

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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  nwg
Protocols & Articles

Articles

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A valuable measure of the knock-down potency of any RNAi experiment is the reduction in protein level. Displayed below are the knock-down rates of  selected esiRNAs using quantitative western blot an...
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Peer-Reviewed Papers
15

References

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