EMAIL THIS PAGE TO A FRIEND
A5960 Sigma

L-Ascorbic acid

BioXtra, ≥99.0%, crystalline

Synonym: L-Threoascorbic acid, Antiscorbutic factor, Vitamin C

Purchase

Properties

Related Categories Biochemicals and Reagents, Carbohydrate Library Collection, Cell Biology, Cofactors, Core Bioreagents,
InChI Key   CIWBSHSKHKDKBQ-JLAZNSOCSA-N
product line   BioXtra
assay   ≥99.0%
form   crystalline
impurities   ≤0.0005% Phosphorus (P)
  ≤0.1% Insoluble matter
ign. residue   ≤0.1%
color   white to off-white
pH   1.0 - 2.5 (25 °C, 176 g/L in water)
mp   190-194 °C (dec.)
solubility   H2O: 0.5 M clear, colorless
anion traces   sulfate (SO42-): ≤0.05%
cation traces   Al: ≤0.0005%
  Ca: ≤0.0005%
  Cu: ≤0.0005%
  Fe: ≤0.0005%
  K: ≤0.005%
  Mg: ≤0.0005%
  NH4+: ≤0.05%
  Na: ≤0.005%
  Pb: ≤0.001%
  Zn: ≤0.0005%
Gene Information   human ... SLC23A2(9962)

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Caution

May darken in storage.

Packaging

10 mg in glass bottle

25, 100, 500 g in poly bottle

Application

• L-Ascorbic acid has been used to induce osteoblast differentiation from primary bone marrow cultures.
• It has been used for the differentiation of hCMPCs (human cardiomyocyte progenitor cells) into cardiomyocytes.
• It has been used to measure the mitochondrial electron transport chain (complex IV) activity.

Biochem/physiol Actions

Ascorbic Acid, also known as Vitamin C, is a six-carbon lactone produced by plants and some animal species but not by humans and other primates. Ascorbic acid functions as an enzymatic cofactor for multiple enzymes, serving as an electron donor for monooxygenases and dioxygenases. Ascorbic acid also functions as a powerful antioxidant, particularly in regards to reactive oxygen species.

Price and Availability


All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
CI7650000

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
Is L-ascorbic acid, Product A5960, the same as vitamin C?
Yes, L-ascorbic acid is a synonym for vitamin C.
What is the solution stability of L-ascorbic acid, Product A5960?
Aqueous solutions are stable only in the absence of oxygen.  Aqueous solutions are most stable at pH 5-6, very unstable at alkaline pH. (Data for Biochemical Research, 3rd ed., Dawson, R. M. C., et al., Oxford University Press (New York, NY: 1986), p. 116)  Degradation is markedly increased in the presence of transition metal ions, especially Cu(II) and Fe(III).  The first stage of oxidation of L-ascorbic acid to dehydroascorbic acid is reversible and the biological activity is retained.  Further oxidation to 2,3-diketogulonic acid is not reversible and the activity is lost. (Omura, H., and Yamafuji, K., in Methodicum Chimicum, 11(2), Korte, F., and Goto, M., eds., Academic Press (New York, NY: 1977), p. 115).
What is the pKa of L-ascorbic acid, Product A5960?
The pKa values for L-ascorbic acid (from the Merck Index) are 4.17 and 11.57.
What is the extinction coefficient of L-ascorbic acid, Product A5960?
According to the Data for Biochemical Research, 3rd ed., Dawson, R. M. C., et al., Oxford University Press (New York, NY 1986), p. 116, the L-ascorbic acid extinction coefficient: EmM = 7.0 (265 nm, water), 7.5 (245 nm, acid).
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
Show more questions
Protocols & Articles
Peer-Reviewed Papers
15

References

Related Products

related product

Product #

Description

Add to Cart

699004 L-Ascorbic acid-2-13C, ≥99 atom % 13C, ≥98% (CP)
699012 L-Ascorbic acid-3-13C, 99 atom % 13C, 98% (CP)

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?