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A5968 Sigma

Anti-AP-1 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-c-Jun

Properties

Related Categories AA - CA, AO-AQ, Alphabetical Index, Antibodies, Antibodies for Gene Regulation,
species reactivity   human
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using formalin-fixed, paraffin-embedded sections of human colon carcinoma
  microarray: suitable
  western blot: 1:200 using HeLa nuclear extract
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt 39 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... JUN(3725)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 246-263 of human c-Jun with N-terminally added lysine, conjugated to KLH.

General description

Activation Protein transcription factor 1 (AP-1) is a leucine zipper transcription factor that recognizes a specific palindromic sequence present in several promoters and enhancers. AP-1 can be induced by several growth and apoptosis-related signaling pathways. Jun proteins are also involved in metastatic, inflammatory and stress responses.
Anti-AP-1/c-Jun recognizes an epitope located on the c-Jun DNA binding domain. This epitope is highly conserved in c-Jun, Jun B and Jun D proteins of chicken, mouse, rat, and human. By immunoblotting, the antibody reacts specifically with AP-1/c-Jun (a single band or occasionally a doublet at 39kDa region). Additional bands of lower molecular weight may be observed. Staining of AP-1/c-Jun band(s) is inhibited by the AP-1/c-Jun immunizing peptide (amino acid residues 246-263).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Application

Immunofluorescence of Hela cells was performed using monoclonal anti-AP1 (clone 100/3) as the primary antibody.

Rabbit Anti-AP-1 antibody can be used for western blot (1:200), immunohistochemistry and microarray applications.

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