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A6063 Sigma

Anti-Horse IgG (whole molecule)−Alkaline Phosphatase antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

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Properties

Related Categories Alkaline Phosphatase, Alphabetical Index, Antibodies, HJ-HO, Horse Secondary Antibodies and Conjugates,
biological source   rabbit
antibody form   affinity isolated antibody
clone   polyclonal
form   buffered aqueous glycerol solution
application(s)   direct ELISA: 1:30,000
  western blot: 1:30,000
conjugate   alkaline phosphatase conjugate
shipped in   wet ice
storage temp.   2-8°C

Description

Immunogen

Horse IgG

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 10 mM glycine, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide

Application

Anti-Horse IgG (whole molecule)-Alkaline Phosphatase antibody is suitable for use in direct ELISA (1:30,000) and western blot (1:30,000).

Binding of horse anti-diphtheria toxin IgG was analyzed by ELISA using alkaline phosphatase-conjugated rabbit anti-horse IgG.

General description

Horse IgGs have seven subclasses ranging from IgG1 to IgG7. Equine IgG antibodies mainly regulate mucosal and systemic immunological responses and thereby, provide protection against disease-causing pathogens such as Streptococcus equi., and the horse flu virus. Horse IgG may also function to control the advancement of EHV-1 infection . Anti-Horse IgG (whole molecule)-Alkaline Phosphatase antibody is specific for IgG in horses.

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

Related Content

Antibody Explorer - Over 80,000 Products

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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