• USA Home
  • A6066 - Anti-Rat IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit

A6066 Sigma

Anti-Rat IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution



Related Categories Alkaline Phosphatase, Alphabetical Index, Antibodies, Primary Antibodies, RAE-RAX,
biological source   rabbit
antibody form   affinity isolated antibody
clone   polyclonal
form   buffered aqueous glycerol solution
application(s)   direct ELISA: 1:30,000
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100
  western blot: 1:30,000-1:60,000
conjugate   alkaline phosphatase conjugate
shipped in   wet ice
storage temp.   2-8°C



Purified rat IgG


Binds all rat Igs.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol, and 15 mM sodium azide.


A concentration of 1:10,000 in direct ELISA and 1:5000 in immunoblotting was used to detect salmon thrombin and fibrinogen in the blood of Wistar rats. The antibody may be used for immunohistochemistry at a concentration of 1:50-1:100 determined by indirect assay using formalin fixed paraffin embedded human tonsil sections and rat Monoclonal Anti-Human IgG as primary antibody.

Anti-Rat IgG (whole molecule)-Alkaline Phosphatase antibody is suitable for use in ELISA. The product can also be used for immunohistochemistry (at 1:50-1:100 dilutions using formalin-fixed, paraffin-embedded sections) and western blot (1:30,000-1:60,000).

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Cell extracts of V. vulnificus strains were analyzed for protein expression by western blot using alkaline phosphatase-conjugated rabbit anti-rat IgG as the secondary antibody at a dilution of 1:1000.

General description

IgGs are glycoprotein antibodies that modulate several immune responses. Rat IgGs have four subclasses, namely, IgG1, IgG2a, IgG2b, and IgG2c. Rat IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rat IgG is a useful tool for the analysis of target proteins. Anti-Rat IgG (whole molecule)-Alkaline Phosphatase antibody binds to all rat immunoglobulins.

The affinity isolated antibody is obtained from anti-rat IgG antiserum by immunospecific purification, which removes essentially all rabbit serum proteins, including immunoglobulins, which do not specifically bind to rat IgG. Anti-Rat IgG is conjugated to alkaline phosphatase by protein cross linking with 0.2% glutaraldehyde
Identity and purity of the antibody is established by immunoelectrophoresis (IEP) prior to conjugation. Electrophoresis of the product followed by diffusion versus anti-rabbit IgG and anti-rabbit whole serum results in single arcs of precipitation.

Price and Availability

Antibody Explorer

Explore with Confidence

Amplified Detection
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Protocols & Articles


How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?