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A6066 Sigma

Anti-Rat IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

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Properties

Related Categories Alkaline Phosphatase, Alphabetical Index, Antibodies, Primary Antibodies, RAE-RAX,
biological source   rabbit
antibody form   affinity isolated antibody
clone   polyclonal
form   buffered aqueous glycerol solution
application(s)   direct ELISA: 1:30,000
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100
  western blot: 1:30,000-1:60,000
conjugate   alkaline phosphatase conjugate
shipped in   wet ice
storage temp.   2-8°C

Description

Immunogen

Purified rat IgG

Specificity

Binds all rat Igs.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol, and 15 mM sodium azide.

Application

A concentration of 1:10,000 in direct ELISA and 1:5000 in immunoblotting was used to detect salmon thrombin and fibrinogen in the blood of Wistar rats. The antibody may be used for immunohistochemistry at a concentration of 1:50-1:100 determined by indirect assay using formalin fixed paraffin embedded human tonsil sections and rat Monoclonal Anti-Human IgG as primary antibody.

Anti-Rat IgG (whole molecule)-Alkaline Phosphatase antibody is suitable for use in ELISA. The product can also be used for immunohistochemistry (at 1:50-1:100 dilutions using formalin-fixed, paraffin-embedded sections) and western blot (1:30,000-1:60,000).

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Cell extracts of V. vulnificus strains were analyzed for protein expression by western blot using alkaline phosphatase-conjugated rabbit anti-rat IgG as the secondary antibody at a dilution of 1:1000.

General description

IgGs are glycoprotein antibodies that modulate several immune responses. Rat IgGs have four subclasses, namely, IgG1, IgG2a, IgG2b, and IgG2c. Rat IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rat IgG is a useful tool for the analysis of target proteins. Anti-Rat IgG (whole molecule)-Alkaline Phosphatase antibody binds to all rat immunoglobulins.

The affinity isolated antibody is obtained from anti-rat IgG antiserum by immunospecific purification, which removes essentially all rabbit serum proteins, including immunoglobulins, which do not specifically bind to rat IgG. Anti-Rat IgG is conjugated to alkaline phosphatase by protein cross linking with 0.2% glutaraldehyde
Identity and purity of the antibody is established by immunoelectrophoresis (IEP) prior to conjugation. Electrophoresis of the product followed by diffusion versus anti-rabbit IgG and anti-rabbit whole serum results in single arcs of precipitation.

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Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Articles

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The following information is provided to help you decide which secondary antibody may be best for your particular application.
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Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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Peer-Reviewed Papers
15

References

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