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A6093 Sigma

Anti-ATM antibody, Mouse monoclonal

clone SYR6D4, purified from hybridoma cell culture

Synonym: Anti-Ataxia-Telangiectasia Mutated, Monoclonal Anti-ATM antibody produced in mouse

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Properties

Related Categories AA - CA, ATH-AZ, ATM, Alphabetical Index, Antibodies,
species reactivity   human
application(s)   immunocytochemistry: suitable
  immunoprecipitation: suitable
  microarray: suitable
  western blot: 1-2 μg/mL using a whole cell extract of an ATM high-producer human melanoma cell line
clone   SYR6D4, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 350 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... ATM(472)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 819-844 of human ATM.

General description

Ataxia-telangiectasia, mutated (ATM) is a protein kinase that may regulate cell cycle and the response to DNA damage. ATM is known to associate with β-adaptin and β-NAP and hence, may modulate intracellular transport of proteins and vesicles. Mouse Monoclonal Anti-ATM antibody recognizes human ATM (approx. 350kDa).

Physical form

Solution 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Endogenous ATM was immunoprecipitated from whole cell lysates using monoclonal anti-ATM (SYR6D4). Immunoprecipitates were used for an ATM kinase assay.

Mouse Monoclonal Anti-ATM antibody can be used for western blot at 1-2μg/mL using a whole cell extracts of melanoma cell lines. The antibody can also be used for microarray, immunocytochemistry and immunoprecipitation assays.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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