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A6132 Sigma

Apyrase from potatoes

ATPase 3.0-60.0 units/mg protein, lyophilized powder

Synonym: Adenosine 5′-diphosphatase, Adenosine 5′-triphosphatase

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Properties

Related Categories 3.6.x.x Acting on acid anhydrides, 3.x.x.x Hydrolases, Biochemicals and Reagents, Cell Biology, Enzyme Class Index,
Quality Level   PREMIUM
type   Grade I
form   lyophilized powder
composition   Protein, 30-60%
storage temp.   −20°C

Description

Application

At least two isoenzymes are found in different varieties of S. tuberosum: one with a high ATPase/ADPase ratio (∼10) and another with a low ratio (∼1).
Reaction: ATP → ADP+Pi → AMP+2Pi.

Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation .

Other Notes

Mixture of high and low ratio isoenzymes.

Packaging

200 units in glass bottle

Sold on the basis of ATPase units.

500, 1000, 2000, 5000 units in poly bottle

Unit Definition

One unit will liberate 1.0 micromole of inorganic phosphorus per minute at pH 6.5 at 30 °C

Physical form

Partially purified, lyophilized powder containing potassium succinate

Biochem/physiol Actions

Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis . Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.

Price and Availability


Available in ELITE Grade

All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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