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A6255 Sigma

α-Amylase from porcine pancreas Green Alternative

DFP Treated, Type I-A, saline suspension, ≥1000 units/mg protein (E1%/280)

Synonym: β-N-acetylglucosaminidase porcine placenta, PPA, al1,4 glucan-4-glucanohydrolase,, porcine pancreas α-amylase

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

General description

Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for waste prevention when used in starch ethanol research. For more information see the article in biofiles.

Molecular mass: 51-54 kDa.
α-Amylase isolated from porcine pancreas is a glycoprotein. It is a single polypeptide chain of ~475 residues containing two SH groups and four disulfide bridges and a tightly bound Ca2+ necessary for stability. Chloride ions are necessary for activity and stability. The pH range for activity is 5.5 to 8.0, with the pH optimum at 7.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Preparation Note

DFP treated. 2× crystallized

Unit Definition

One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 6.9 at 20 °C.

Physical form

Suspension in 2.9 M NaCl solution containing 3 mM CaCl2

Application

α-Amylase is used to hydrolyze α bonds of α-linked polysaccharides, such as starch and glycogen. Product A6255 is from porcine pancreas and is type I-A. α-Amylase, from Sigma, has been used in various plant studies, such as metabolism studies in Arabidopsis .

Biochem/physiol Actions

α-Amylase hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose. α -Amylase, from porcine pancreas, is a glycoprotein that consists of a single polypeptide chain of approximately 475 residues containing 2 SH groups and four disulfide bridges and a tightly bound Ca2+ necessary for stability.

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Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
BU7432500

Frequently Asked Questions

How does the storage temperature relate to shipping conditions?
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. http://www.sigmaaldrich.com/etc/medialib/docs/Sigma-Aldrich/General_Information/shippingandlong-termstoragev3.Par.0001.File.tmp/shippingandlong-termstoragev3.pdf
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
How should Product No. A6255, α-Amylase from porcine pancreas, be dissolved?
The product is soluble in water (1 mg/mL), yielding a clear, colorless solution.  This information can be found on the Product Information Sheet:  http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Product_Information_Sheet/a6255pis.Par.0001.File.tmp/a6255pis.pdf
What does "DFP treatment" refer to for Product No. A6255, α-Amylase from porcine pancreas?
Diisopropylfluorophosphate (DFP) is a potent irreversible inhibitor of serine proteases, such as trypsin, chymotrypsin, and acetylcholinesterase.
What is the solution stablity of Product No. A6255, α-Amylase from porcine pancreas?
α-Amylase is stable in 25 mM Tris-HCl, pH 7.5, with 100 mM KCl, refrigerated at near 0 °C or frozen at -20 °C for at least 9 days. Another recommended storage condition is in 1 mM phosphate, pH 7.3, with 30 mM CaCl2 at -15 °C.  
How is Product No. A6255, α-Amylase from porcine pancreas, used?
α-Amylase is an enzyme that breaks down polysaccharides, such as starch and glycogen, to give glucose and maltose. α-Amylase hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. The α-(1,6) bond is not hydrolyzed. The following link provides the Sigma-Aldrich α-Amylase enzymatic test assay:  http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Enzyme_Assay/a6255enz.Par.0001.File.tmp/a6255enz.pdf
What is the recommended substrate for the α-Amylase test of Product No. A6255?
The recommended substrate for the enzymatic α-Amylase test is Product No. S2630, Starch.
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Protocols & Articles

Protocols

Enzymatic Assay of α-Amylase (EC 3.2.1.1)

The spectrophotometric stop reaction determination (A540, Light path = 1 cm) is based on the following reaction:

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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