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A6279 Sigma

Aprotinin from bovine lung

saline solution, 3-7 TIU/mg protein

Synonym: Trasylol, Trypsin inhibitor (basic)

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Description

Application

Aprotinin is largely used as an inhibitor of trypsin.

Caution

Aprotinin products should be stored at 2-8°C.

Preparation Note

This product is an aseptically filled solution in 0.9% NaCl and 0.9% benzyl alcohol. It has an activity of 3-7 TIU/mg protein and 5-10 TIU/mL solution.

Unit Definition

One Trypsin Inhibitor Unit (TIU) will decrease the activity of two trypsin units by 50%, where one trypsin unit will hydrolyze 1.0 μmole of N-alpha-benzoyl-DL-arginine p-nitroanilide per minute at pH 7.8 and 25°C. Another commonly used unit is the KIU, with 1 TIU = 1,300 KIU.

Biochem/physiol Actions

Aprotinin is a competitive serine protease inhibitor that forms stable complexes with and blocks the active sites of enzyme. This binding is reversible, and most aprotinin-protease complexes will dissociate at extreme pH levels >10 or <3. Structurally, Aprotinin is a monomeric globular protein derived from bovine lung that consists of 58 amino acids, arranged in a single polypeptide chain with three crosslinking disulfide bridges.

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Detect Your Unseen Protein

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Amicon Ultra Centrifugal Filters
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg
Protocols & Articles

Protocols

Enzymatic Assay of Aprotinin

The scope of this procedure is all products that have a specification for Aprotinin activity.
Keywords: Enzymology, Extinction coefficient

Peer-Reviewed Papers
15

References

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