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A6284 Sigma

Human IgG−Agarose

saline suspension

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Properties

Related Categories Adsorption Resins, Antibodies, Antibody Purification and Characterization, Supplementary Products
form   saline suspension
extent of labeling   ≥5 mg per mL
matrix   Cross-linked 4% beaded agarose
matrix activation   cyanogen bromide
matrix spacer   1 atom
conjugate   agarose conjugate
storage temp.   2-8°C

Description

Physical form

Suspension in 0.5 M NaCl containing 0.01% thimerosal

Application

IgG-Agarose is an immuno-adsorbent that can be used to affinity purify antibodies, remove species-specific cross-reacting antibodies or remove contaminating antibodies from an antiserum preparation. Typically, cross-reacting antibodies may be removed from an antiserum preparation using an equal volume of IgG-Agarose (resin volume). However, the resin to antiserum ratio will vary with individual applications.

Tandem affnitiy purification pulldown was performed in yeast cultures using agarose-linked human IgG. Supernatants were mixed with 30μl of agarose beads overnight.

General description

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders.

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Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

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Peer-Reviewed Papers
15

References

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